Determination of chemical composition, in vitro dry matter digestibility and in vitro gas production of forage by near-infrared reflectance spectroscopy

Authors

  • Andrej LAVRENČIČ Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia
  • Bruno STEFANON Univ. of Udine, Veterinary Fac., Dept. of Animal Sciences, Via delle Scienze 208, I-33100 Udine, Italy
  • Barbara TOSO Univ. of Udine, Veterinary Fac., Dept. of Animal Sciences, Via delle Scienze 208, I-33100 Udine, Italy
  • Piero SUSMEL Univ. of Udine, Veterinary Fac., Dept. of Animal Sciences, Via delle Scienze 208, I-33100 Udine, Italy

DOI:

https://doi.org/10.14720/aas.2001.78.2.15603

Keywords:

animal nutrition, forage, chemical composition, in vitro dry matter digestibility, in vitro gas production, analytical chemistry, near infrared reflectance spectroscopy (NIRS)

Abstract

One hundred dried samples of forage, harvested three or four times during 1997 and 1999 in different morphological stages were analysed for chemical composition, in vitro dry matter digestibility (IVDMD; Tilley and Terry, 1963) and in vitro gas production (Menke and Steingass, 1988) by laboratory methods and by near infrared reflectance spectroscopy (NIRS). NIRS predicted the main chemical components, such as crude protein, crude fibre, neutral detergent fibre, acid detergent fibre, with a very high degree of accuracy (i.e. the determination coefficient (R2) varied from 93.5 % for ADF to 98,2 % for NDF), while the coefficients of determination of crude ash, ether extract and acid detergent lignin were 79.6, 85.1 and 82.3 %, respectively, with standard errors of cross validation (SECV) exceeding 9.0 %. The IVDMD was also determined with a high degree of accuracy (R2 = 94.3 %; SECV = 4.3 %), such as parameters of gas production "B" (total gas production) and "A" (constant factor describing the decay in specific gas production rate), estimated with the Gompertz model, which have R2 and SECV values of 93.0 and 3.8 % and 94.5 and 8.0 %, respectively. The parameter "C" (the specific fermentation rate) was predicted with a somewhat lower degree of accuracy (R2 = 78.3 and SECV = 9.6 %).

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Published

15. 12. 2001

Issue

Section

Original Scientific Article

How to Cite

LAVRENČIČ, A., STEFANON, B., TOSO, B., & SUSMEL, P. (2001). Determination of chemical composition, in vitro dry matter digestibility and in vitro gas production of forage by near-infrared reflectance spectroscopy. Acta Agriculturae Slovenica, 78(2), 151–160. https://doi.org/10.14720/aas.2001.78.2.15603

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