The effect of different rapid methods for isolation of Salmonella genomic DNA on PCR sensitivity

Abstract

The purpose of our research was to determine the effect of rapid methods for isolation of Salmonella genomic DNA on PCR sensitivity. Salmonella specific primers ST11 and ST15 were used for DNA amplification. Among tested procedures the best PCR sensitivity was achieved after treating of sample with NaOH and SDS solutions and heating of sample in water bath at 95^o C for 15 min. PCR detection limit for Salmonella in pure and mixed cultures was in a range from 10⁴ to 10⁵ cfu ml^-1. Described procedure for DNA isolation is rapid and not work-consuming.