Molecular cloning of the rRNA genes of Prevotella bryantii B14

Authors

  • Matjaž PETERKA Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia
  • Gorazd AVGUŠTIN Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2000.76.1.15791

Keywords:

microbiology, bacteria, Prevotella bryantii, molecular genetics, ribosomal RNA operons, cloning, rumen

Abstract

Parts of the rRNA operons of anaerobic rumen bacteria Prevotella bryantii B14 were cloned. 350 colonies carrying pBluescriptII with HindIII DNA fragments were screened with 16S rDNA specific probe and six positive clones were obtained. Inserts with 16S rRNA genes were identified by hybridisation with digoxigenin labelled Prevotella-Bacteroides 16S rRNA specific probe. All six inserts were approximately 2 kb long and contained large parts of the 16S rRNA gene, 16S-23S rRNA internal spacer region and approximately 100 bp of 23S rRNA gene. The origin of 16S rRNA genes were confirmed by PCR with P. bryantii 16S rRNA specific primer.

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Published

15. 05. 2000

Issue

Section

Original Scientific Article

How to Cite

PETERKA, M., & AVGUŠTIN, G. (2000). Molecular cloning of the rRNA genes of Prevotella bryantii B14. Acta Agriculturae Slovenica, 76(1), 123–129. https://doi.org/10.14720/aas.2000.76.1.15791

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