Isolation and characterization of bacteriophages of rumen bacterium Prevotella bryantii TC1-1

Authors

  • Darja FERME Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale and present address: Cesta Ceneta Štuparja 140, SI-1231 Ljubljana Črnuče, Slovenia
  • Jerneja AMBROŽIČ Univ. of Ljubljana, Biotechnical Fac., Biology Dept., Večna pot 111, SI-1000 Ljubljana, Slovenia
  • Miklavž GRABNAR Univ. of Ljubljana, Biotechnical Fac., Biology Dept., Večna pot 111, SI-1000 Ljubljana, Slovenia
  • Maja RAVNIKAR National Institute for Biology, Večna pot 111, SI-1000 Ljubljana, Slovenia
  • Gorazd AVGUŠTIN Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.2000.76.2.15814

Keywords:

microbiology, bacteriophage, bacteria, isolation, characterization, TEM, rumen

Abstract

Sampling, inoculation and extraction procedures were elaborated for the isolation of bacteriophages from cattle rumen fluid. Several strains from four different species of rumen bacterial genus Prevotella were used as indicator organisms as well as Escherichia coliSalmonella sp. and Yersinia enterocolitica strains. Only one strain, i.e. P.bryantii TC1-1 was successfully infected with filter sterilised rumen fluid inoculum containing rumen bacteriophages. Two plaque morphotypes were observed, both being turbid and rather small but clearly different in size. The preliminary transmission electron microscopy analysis showed that all observed bacteriophages have presumably icosahedral symmetry. The infection trials of other Prevotella strains with isolated bacteriophages from the strain TC1-1 were successful only with type strains of species P.bryanti and P.brevis respectively. Bacteriophage purification and long term storage procedures were elaborated.

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Published

15. 11. 2000

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Section

Original Scientific Article

How to Cite

FERME, D., AMBROŽIČ, J., GRABNAR, M., RAVNIKAR, M., & AVGUŠTIN, G. (2000). Isolation and characterization of bacteriophages of rumen bacterium Prevotella bryantii TC1-1. Acta Agriculturae Slovenica, 76(2), 191–197. https://doi.org/10.14720/aas.2000.76.2.15814

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