Detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatography

Authors

  • Helena ŠIRCELJ University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia
  • Franc BATIČ University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia

Keywords:

ascorbic acid, photosinthetic pigments, high performance liquid chromatography, oxidative stress, ozone, drought, Malus domestica, Trifolium repens

Abstract

Ascorbic acid and photosynthetic pigments content in leaves of the apple tree (Malus domestica Borkh.) subjected to drought and white clover (Trifolium repens L.) exposed to high ozone concentrations were measured by high performance liquid chromatography in order to detect oxidative stress. Measurements showed a significant difference between stressed and control plants. Water deficit increased ascorbic acid and zeaxanthin, and decreased total chlorophyll (a+b) and b-carotene in apple tree leaves. High concentrations of ozone increased ascorbic acid, total carotenoids, neoxanthin, zeaxanthin and b-carotene, and decreased violaxanthin and antheraxanthin in white clover leaves. Observed changes in ascorbic acid and photosynthetic pigment content in leaves of tested apple tree and white clover plants indicate that oxidative stress may have occured under stress conditions used in our experiments.

Published

15. 09. 1998

Issue

Section

Original Scientific Article

How to Cite

ŠIRCELJ, H., & BATIČ, F. (1998). Detecting oxidative stress in leaves of apple tree and white clover by analysis of ascorbic acid and pigments using high performance liquid chromatography. Acta Agriculturae Slovenica, 71(1), 57–64. https://journals.uni-lj.si/aas/article/view/16014

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