On the localization of xylanolytic enzymes in Prevotella Bryantii B14

Authors

  • Romana MARINŠEK-LOGAR Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia
  • Franc Viktor NEKREP Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.1997.70.1.16053

Keywords:

rumen, anaerobic bacteria, Prevotella bryantii, xylanases, enzyme localization

Abstract

Prevotella bryantii B14 is a strictly anaerobic, Gram-negative, polysaccharides-degrading ruminal bacterium. EDTA/sphaeroplasting and osmotic shock procedure were used in present work to localise endoxylanolytic, b -xylosidase and a -L-arabinofuranosidase activities of P. bryantii B14. Late exponential phase cells released most of the endoxylanolytic and CMC-ase activity by osmotic shock, showing the periplasmic location of both enzymatic activities, while b -xylosidase and a -L-arabinofuranosidase activities were recovered largely in membrane cell fraction. About 23 % of the total culture endoxylanase activity and about 30 % of the CMC-ase activity were found to be extracellular. No b -xylosidase and a -L-arabinofuranosidase activities were detected in culture supernatant of P. bryantii B14.

Published

15. 12. 1997

Issue

Section

Original Scientific Article

How to Cite

MARINŠEK-LOGAR, R., & NEKREP, F. V. (1997). On the localization of xylanolytic enzymes in Prevotella Bryantii B14. Acta Agriculturae Slovenica, 70(1), 31–38. https://doi.org/10.14720/aas.1997.70.1.16053

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