Specific detection of bacterial species in the gut of domestic animals with in situ hybridization and epifluorescent microscopy

Authors

  • Katarina TEPŠIČ Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia
  • Gorazd AVGUŠTIN Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia

Keywords:

in situ hybridization, epifluorescent microscopy, rumen, gut, microorganisms, gram negative bacteria, Prevotella

Abstract

Bacterial cells belonging to the species Prevotella bryantii were specifically detected in rumen samples from a black and white Frisian cow using a narrowly specific rRNA probe PBB1and in situ hybridization combined with epifluorescent microscopy. Bacterial cells from the phylogenetic group cytophaga-flexibacter-bacteroides were also specifically detected with the broad rRNA probe PREBAC. Both probes, labelled with tertramethylrodamine isothiocyanate (TRITC) proved to be highly specific and give strong and clear signal without any or very little of nonspecific fluorescence. The positively identified cells were 2–3 times smaller, when compared to the cells of the laboratory strain P. bryantii B14, but they do exhibit a similar intensity of the signal.

Published

15. 12. 1997

Issue

Section

Original Scientific Article

How to Cite

TEPŠIČ, K., & AVGUŠTIN, G. (1997). Specific detection of bacterial species in the gut of domestic animals with in situ hybridization and epifluorescent microscopy. Acta Agriculturae Slovenica, 70(1), 47–53. https://journals.uni-lj.si/aas/article/view/16055

Similar Articles

1-10 of 258

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)

1 2 > >>