Rumen gas kinetics: a comparative analysis of two in vitro assessment methods for forage evaluation
DOI:
https://doi.org/10.14720/aas.2024.120.1.16702Keywords:
animal nutrition, rumen, gas production, gas kinetic parameters, Ankom RF Gas Production System, Hohenheim Gas TestAbstract
Gas production from thirty samples of feedstuffs (10 samples of corn silage, grass silage, and grass hay, respectively) was assessed in vitro using two methods: the Hohenheim gas test (HGT) and the ANKOM RF Gas Production System (ANKOM). Samples were incubated in buffered rumen fluid. Gas kinetic parameters were calculated using the Gompertz model. Results revealed significantly lower gas production with the ANKOM compared to the HGT. Significant differences were observed between the HGT and ANKOM in the specific gas production rate (parameter C), maximum fermentation rate (MFR) and gas produced after 24 h of incubation (Gas24) for each feed group. High coefficients of determination (R2) were calculated between the methods for the gas kinetic parameters MFR, Gas24, total potential gas production (parameter B), decrease in the specific gas production rate (parameter A), moderate R2 for C, and low R2 for time of maximum fermentation rate (TMFR). Despite the lower quantities of gas generated with the ANKOM, there are strong correlations in the parameters of gas kinetics that promise the possibility of developing correction models. Future development of such models could position the ANKOM as a viable alternative to HGT, particularly for calculating metabolizable energy and net energy for lactation in feedstuffs.
Metrics
Downloads
References
Aiple, K. P., Steingass, H., Drochner, W. (1995). Schätzung des Energiegehaltes von Grunfutteramitteln mit Pepsin-Cellulase-methode und dem Hohenheimer Futterwerttest. Proceedings of the Society of Nutrition Physiology, 4, 99.
Bachmann, M., Kuhnitzsch, C., Martens, S. D., Steinhöfel, O., Zeyner, A. (2020). Estimation of gas production and post-ruminal crude protein from native or ensiled Pisum sativum and Vicia faba grains. Journal of Applied Animal Research, 48(1), 78–85. https://doi.org/10.1080/09712119.2020.1733581
Cattani, M., Tagliapietra, F., Maccarana, L., Hansen, H. H., Bailoni, L., Schiavon, S. (2014). Technical note: In vitro total gas and methane production measurements from closed or vented rumen batch culture systems. Journal of Dairy Science, 97(3), 1736–1741. https://doi.org/10.3168/jds.2013-7462
Cornou, C., Storm, A. I., Hindrichsen, B. I., Worgan, H., Bakewell, C. E., Yáñez-Ruiz, D., … Hansen, H. (2013). A ring test of a wireless in vitro gas production system. Animal Production Science, 53(6), 585–592. https://doi.org/10.1071/AN12091
Davies, Z. S., Mason, D., Brooks, A. E., Grif, G. W., Merry, R. J., Theodorou, M. K. (2000). An automated system for measuring gas production from forages inoculated with rumen fluid and its use in determining the effect of enzymes on grass silage. Animal Feed Science and Technology, 83(3–4), 205–221. https://doi.org/10.1016/S0377-8401(99)00138-8
DLG (Deutsche Ladwirtschafts-Gesellschaft), (1997). Feed Value Table for Ruminants. 7th Revised and Extended Edition (in German: DLG Futterwerttabellen: Wiederkäuer). DLG-Verlag. Frankfurt (Germany)
Elberg, K., Steuer, P., Habermann, U., Lenz, J., Nelles, M., Südekum, K.-H. (2018). A small scale in vitro system for high throughput gas production analysis – A comparison with the Hohenheim gas test. Animal Feed Science and Technology, 241, 8–14. https://doi.org/10.1016/j.anifeedsci.2018.04.001
Getachew, G., Robinson, P. H., DePeters, E. J., Taylor, S. J. (2004). Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds. Animal Feed Science and Technology, 111(1–4), 57–71. https://doi.org/10.1016/S0377-8401(03)00217-7
Gierus, M., Schiborra, A., Südekum, K.-H., Rave, G., Taube, F. (2008). Comparison of gas accumulation profiles of several feeds using manual or automated gas production methods. Animal Feed Science and Technology, 147(4), 310–325. https://doi.org/10.1016/j.anifeedsci.2008.02.001
Goering, H. K., Van Soest, P. J. (1970). Forage Fiber Analysis (Apparatus Reagents, Procedures and Some Applications). Agricultural Handbook No. 397. United States Department of Agriculture, Washington DC.
Lavrenčič A., Stefanon, B., Susmel, P. (1997). An evaluation of the Gompertz model in degradability studies of forage chemical components. Animal Science, 64, 423–431. https://doi.org/10.1017/S1357729800016027
Lowman, R. S., Brooks, A. E., Theodorou, M.-K., Dhanoa, M. S., Cuddeford, D. (1998). The effect of head-space pressure on gas production profiles as determined using the pressure transducer technique. In vitro techniques for measuring nutrient supply to ruminants (ed. Deaville, E. R., Owen, E., Adesogan, A. T., Rymer, C., Huntington, J. A., Lawrence, T. L. J.), pp. 205–206. British Society of Animal Science occasional publication no. 22. https://doi.org/10.1017/S0263967X00032602
Menke K. H., Steingass H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development, 28, 7–55.
Rymer, C., Huntington, J. A., Williams, B. A., Givens, D. I. (2005). In vitro cumulative gas production techniques: History, methodological considerations and challenges. Animal Feed Science and Technology, 123–124, 9–30. https://doi.org/10.1016/j.anifeedsci.2005.04.055
Tagliapietra, F., Cattani, M., Bailoni, L., Schiavon, S. (2010). In vitro rumen fermentation: Effect of headspace pressure on the gas production kinetics of corn meal and meadow hay. Animal Feed Science and Technology, 158(3–4), 197–201. https://doi.org/10.1016/j.anifeedsci.2010.04.003
Theodorou, M. K., Williams, B. A., Dhanoa, M. S., McAllan, A. B., France, J. (1994). A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology, 48(3–4), 185–197. https://doi.org/10.1016/0377-8401(94)90171-6
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Nejc VALCL, Andrej LAVRENČIČ
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
