CHITINOLYTIC ENZYMES IN THE RUMEN MICROBIAL ECOSYSTEM

Authors

  • J. KOPEČNÝ Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Prague 10, Uhříněves, 104 00, Czech Republic
  • Blanka HODROVÁ Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Prague 10, Uhříněves, 104 00, Czech Republic

DOI:

https://doi.org/10.14720/aas.1998.72.1.17077

Keywords:

microbiology, bacteria, Clostridium, fungi, enzymes, chitinolysis, rumen

Abstract

Chitinolytic systems of rumen Clostridia (usually the most active rumen chitinolytic bacteria) and rumen anaerobic fungi were compared in the present study. The chitinolytic enzymes of tested Clostridia strains were represented mainly by extracellular exochitinases, N-acetylglucosaminidases, chitin deacetylases and chitosanases. Zymograms of exochitinases revealed that these enzymes were bound in complexes with MW higher than 100 kDa. Final products of chitin degradation were glucosamine, N-acetylglucosamine and chitin oligosaccharides. Fungal chitinases were represented mainly by extracellular endochitinases and chitin deacetylase in both mono- and polycentric fungi. The main activities in cellular fraction were chitosanase and endochitinase. There were no or low activity of N-acetylglucosaminidase activity in all fungal strains tested. In comparison with rumen chitinolytic bacteria there was lower production of exochitinases and no production of N-acetylglucosaminidase in rumen fungi.

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Published

15. 12. 1998

Issue

Section

Original Scientific Article

How to Cite

KOPEČNÝ, J., & HODROVÁ, B. (1998). CHITINOLYTIC ENZYMES IN THE RUMEN MICROBIAL ECOSYSTEM. Acta Agriculturae Slovenica, 72(1), 85–93. https://doi.org/10.14720/aas.1998.72.1.17077

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