MOLECULAR GENOTYPING OF RUMEN FUNGI BASED ON RFLP ANALYSIS

Authors

  • Kateřina FLIEGEROVÁ Academy of Sciences of Czech Republic, Institute of Animal Physiology and Genetics, Prague 10, Uhříněves, 104 00, Czech Republic
  • Sylva PAŽOUTOVÁ Academy of Sciences of Czech Republic, Institute of Microbiology, Prague 4, 140 00, Czech Republic
  • Blanka HODROVÁ Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Prague 10, Uhříněves, 104 00, Czech Republic

DOI:

https://doi.org/10.14720/aas.1998.72.1.17078

Keywords:

microbiology, anaerobic fungi, molecular genetics, RFLP, classification, rumen

Abstract

Eight polycentric strains of the genera Orpinomyces and five monocentric strains of the genera Neocallimastix were examined for the restriction fragment length polymorphisms. EcoRI-digested nuclear DNA of isolates of rumen fungi and DNA-DNA hybridization with probe RS2 revealed several distinct cleavage profiles. Results are ambiguous and interpretation is difficult with respect to the high genetic variability of rumen fungi isolates.

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Published

15. 12. 1998

Issue

Section

Original Scientific Article

How to Cite

FLIEGEROVÁ, K., PAŽOUTOVÁ, S., & HODROVÁ, B. (1998). MOLECULAR GENOTYPING OF RUMEN FUNGI BASED ON RFLP ANALYSIS. Acta Agriculturae Slovenica, 72(1), 95–98. https://doi.org/10.14720/aas.1998.72.1.17078

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