ANALYSIS OF COMPLEX MICROBIAL ECOSYSTEMS WITH IN SITU HYBRIDIZATION AND EPIFLUORESCENT MICROSCOPY

Authors

  • Katarina TEPŠIČ Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia
  • G. AVGUŠTIN Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.1998.72.1.17079

Keywords:

microbiology, bacteria, Prevotella, molecular biology, In situ hybridization, epifluorescent microscopy, rumen

Abstract

In situ hybridization with fluorescent oligonucleotide probes combined with epifluorescent microscopy was used to detect and localize microorganisms in rumen samples from a black and white Friesian cow. Bacterial cells were hybridized with 16S rRNA targeted oligonucleotide probes specific for the phylogenetic group Cytophaga-Flexibacter-Bacteroides (CFB) (BACPRE) and for species Prevotella bryantii B14 (PBB14) and P.ruminicola 23T (PR23). The oligonucleotide probes were labelled with fluorescine isothiocyanate (FITC) or tetramethylrodamine isothiocyanate (TRITC). Both species specific probes proved to be highly specific and gave strong and clear signal.

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Published

15. 12. 1998

Issue

Section

Original Scientific Article

How to Cite

TEPŠIČ, K., & AVGUŠTIN, G. (1998). ANALYSIS OF COMPLEX MICROBIAL ECOSYSTEMS WITH IN SITU HYBRIDIZATION AND EPIFLUORESCENT MICROSCOPY. Acta Agriculturae Slovenica, 72(1), 99–103. https://doi.org/10.14720/aas.1998.72.1.17079

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