GENETIC CHARACTERIZATION OF LF221 ACIDOCINS

Authors

  • Andreja ČANŽEK MAJHENIČ Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia
  • Irena ROGELJ Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, Slovenia

DOI:

https://doi.org/10.14720/aas.1998.72.1.17080

Keywords:

microbiology, bacteria, Lactobacillus acidophilus, bacteriocins, molecular genetics, cloning, classification

Abstract

Acidocin LF221 A and B are at least two different bacteriocins produced by Lactobacillus acidophilus LF221. In previous research work the N-terminal amino acid (AA) sequence was determined from the purified peptides and probes for both acidocins were prepared as well. Southern hybridization was used to locate structural genes for acidocin LF221 A and B on the genome. Using as a template digested chromosomal DNA of either LF221 or its bac- derivative, identical results were obtained. A possible explanation for this is that bac- mutant still contains the structural genes for both acidocins. When acidocin LF221 A or B were compared to other known bacteriocins, they showed some homology to ThmB peptide of thermophilin 13 or LafX peptide of lactacin F. We assume that LF221 acidocins are novel representatives of the class II bacteriocins.

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Published

15. 12. 1998

Issue

Section

Original Scientific Article

How to Cite

ČANŽEK MAJHENIČ, A., & ROGELJ, I. (1998). GENETIC CHARACTERIZATION OF LF221 ACIDOCINS. Acta Agriculturae Slovenica, 72(1), 105–112. https://doi.org/10.14720/aas.1998.72.1.17080