Nephroprotective Effect of Methanol Extract of Crassocephalum Crepidioides (Benth.) S. Moore (Ebolo) during Paracetamol- Induced toxicity in Wistar Rats
Keywords:Antioxidants, C. crepidioides, rat kidney, malondialdehyde, paracetamol, toxicity, detoxification, drug
AbstractThe effect of Crassocephalum crepidioides (Benth.) S. Moore against Paracetamol (PCM)-induced oxidative stress was investigated. Wistar rats were divided into five groups of six rats. The control was group 1, Groups 2 and 3 were administered 250 mg/kg/bwt PCM and 300 mg/kg/bwt methanol extract of C. crepidioides leaves (MECL) respectively, group 4 and 5 were co-administered with 250 mg/kg/bwt PCM + 300 mg/kg/bwt MECL and 250 mg/kg/bwt PCM + 50 mg acetylcysteine (NAC) respectively for 2 weeks, following 1week pre-administration with 300 mg/kg/bwt MECL and 50mg NAC respectively. Kidney damage was measured by evaluating serum urea and creatinine, while antioxidant status was assessed by evaluating glutathione (GSH) level, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase activities. Lipid peroxidation (LPO) was determined from malondialdehyde (MDA) levels. PCM had no significant effect on serum urea and creatinine levels, but significantly decreased glutathione levels, SOD and catalase activities, while the activity of glutathione-S-transferase and level of malondialdehyde (MDA) was increased significantly. Co-administration with MECL or NAC reversed these effects. In conclusion, C. crepidioides. protects against PCM-induced oxidative stress in the Kidneys of Wistar rats.
Adedayo B C, Oboh G, Oyeleye S I, Tech M, Ejakpovi I I, Boligon, A A, Athayde M L (2015) Blanching alters the phenolic constituents and in vitro antioxidant and anticholinesterases properties of fireweed (Crassocephalum crepidioides). Journal of Taibah University Medical Sciences 10 (4):419-426. DOI: https://doi.org/10.1016/j.jtumed.2015.09.003
Ahmad S T, Arjumand W, Nafees S (2012) Hesperidin alleviates acetaminophen-induced toxicity in Wistar rats by abrogation of oxidative stress, apoptosis and inflammation. Toxicol Lett 208:149–161. DOI: https://doi.org/10.1016/j.toxlet.2011.10.023
Arakawa S (2013) Utilization of glutathione S-transferase Mu 1-and Theta 1-null mice as animal models for absorption, distribution, metabolism, excretion and toxicity studies. Expert Opin Drug Metab Toxicol 9 (6):725–736. DOI: https://doi.org/10.1517/17425255.2013.780027
Arawande J O, Komolafe E A, Imokhuede B (2013) Nutritional and phytochemical compositions of fireweed (Crassocephalum crepidioides). Journal of Agricultural Technology 9 (2):439-449.
Bahar E, Siddika M S, Nath B, Yoon H (2016) Evaluation of in vitro Antioxidant and in vivo Antihyperlipidemic Activities of Methanol Extract of Aerial Part of Crassocephalum crepidioides (Asteraceae) Benth S Moore. Tropical Journal of Pharmaceutical Research 15 (3): 481-488. DOI: https://doi.org/10.4314/tjpr.v15i3.8
Bateman D N, Dear J W (2019) Acetylcysteine in paracetamol poisoning: a perspective of 45 years of use. Toxico Res 8:489-498. DOI: https://doi.org/10.1039/C9TX00002J
Bello M O, Ogbesejana B A, Uduma U A (2019) Antioxidant and Lipoxygenase Inhibitory Activity of Crassocephalum crepidioides (Benth) S Moore. an Underutilized Vegetable from Nigeria. FRsCS 1 (2):28-31
Bello O M, Zaki A A, Khan I S, Fasinu P S, Ali Z, Khan I A, Usman L A, Oguntoye O S(2017) Assessment of selected medicinal plants indigenous to West Africa for antiprotozoal activity. South African Journal of Botany DOI: 10.1016/JSAJB.2017. DOI: https://doi.org/10.1016/j.sajb.2017.08.002
Beutler E Duron O, Kelly B M (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882-888.
Buege J A, AustS D (1978) “Microsomal lipid peroxidation methods. ”Enzymol 52:302–310. DOI: https://doi.org/10.1016/S0076-6879(78)52032-6
Canayakin D, Bayir Y, Baygutalp K N Karaoglan E S, Atmaca H T, Kocak Ozgeris F B, Keles M S, Halici Z(2016). Paracetamol-induced nephrotoxicity and oxidative stress in rats: the protective role of Nigella sativa. Pharmaceutical Biology 54 (10):1-10. DOI: https://doi.org/10.3109/13880209.2016.1145701
Chiam E, Weinberg, L, Bellomo R (2015). Paracetamol: a review with a specific focus on the haemodynamic effects of intravenous administration. Heart, Lung and Vessels 7(2):121-132.
Chinnappan S M, George A, Thaggikuppe K P, Choudhary Y, Choudhary V K, Ramani Y, Dewangan R(2019). Nephroprotective Effect of Herbal Extract Eurycoma longifolia on Paracetamol-Induced Nephrotoxicity in Rats. Evidence-Based Complementary and Alternative Medicine DOI :10.1155/2019/4916519. DOI: https://doi.org/10.1155/2019/4916519
Das J, Ghosh J, Manna P, Kuma B S (2010) Taurine protects acetaminophen-induced oxidative damage in mice kidney through APAP urinary excretion and CYP2E1 inactivation. Toxicology 269: 24–34. DOI: https://doi.org/10.1016/j.tox.2010.01.003
Dasari S, Ganjayi M S, Yellanurkonda P, Basha S, Meriga B (2018) Role of glutathione S-transferases in detoxification of a polycyclic aromatic hydrocarbon; methylcholanthrene. Chemico-Biological Interactions 294:81-90. DOI: https://doi.org/10.1016/j.cbi.2018.08.023
Demirba S, Uysal B, Guven A (2010) Effects of medical ozone therapy on acetaminophen-induced nephrotoxicity in rats. Ren Fail 32:493–497. DOI: https://doi.org/10.3109/08860221003646352
El-Shafey, M M Abd-Allah, G M Mohamadin, A M Harisa, G I, Mariee A D(2015) Quercetin protects against acetaminophen-induced hepatorenal toxicity by reducing reactive oxygen and nitrogen species. Pathophysiology 22:49–55. DOI: https://doi.org/10.1016/j.pathophys.2014.12.002
Habig W H, Pabst M J, Jakoby W B (1974) Glutathione S-transferases; “The first enzymatic step in mercapturic acid formation. Journal of biological chemistry 249:7130-7139. DOI: https://doi.org/10.1016/S0021-9258(19)42083-8
Hasna T, Hasnaa Y, Weiwei X, Changxin W, Rongshan L, Yafeng L (2021) Mitochondrial Reactive Oxygen Species and Their Contribution in Chronic Kidney Disease Progression Through Oxidative Stress. Frontiers in Physiology 12: DOI: 10.3389/fphys.2021.627837 DOI: https://doi.org/10.3389/fphys.2021.627837
Hernandez E P, K K Talactac, M R Galay, R L Hatta, T Fujisaki, K Tsuji, N Tanaka T (2018). Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis. Parasites & Vectors 11:460. DOI: https://doi.org/10.1186/s13071-018-3044-9
Ishitsuka Y,Kondo Y, KadowakiD(2020).Toxicological Property of Acetaminophen: The Dark Side of a Safe Antipyretic/Analgesic Drug. Biol Pharm Bull 43 (2):195-206. DOI: https://doi.org/10.1248/bpb.b19-00722
Kale R H, Halde U K, Biyani K R (2012) Protective Effect of Aqueous Extract of Uraria Picta on Acetaminophen Induced Nephrotoxicity in Rats. International Journal of Research in Pharmaceutical and Biomedical Sciences 3:110-113.
Kim S Y, Moon A (2012) Drug-induced nephrotoxicity and its biomarkers. Biomolecules & Therapeutics 20:268–272. DOI: https://doi.org/10.4062/biomolther.2012.20.3.268
Lowry O H, Rosebrough N J, Farr A L, Randall R J(1951) Protein measurement with the Folin Phenol Reagent”. Journal of Biological Chemistry 193:269-275. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6
Mazer M, Perrone J (2008) Acetaminophen-induced nephrotoxicity: pathophysiology, clinical manifestations, and management. Journal of medical toxicology 4 (1):2-6. DOI: https://doi.org/10.1007/BF03160941
Mazaleuskaya L, L Sangkuhl, K Thorn, C F FitzGerald, G A Altman, R B, Klein T E(2015) PharmGKB summary: Pathways of acetaminophen metabolism at the therapeutic versus toxic doses. Pharmacogenet Genomics 25 (8):416–426. DOI: https://doi.org/10.1097/FPC.0000000000000150
Misra H P, Fridovich, I (1972) The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. Journal of biological chemistry 247:3170-3175 DOI: https://doi.org/10.1016/S0021-9258(19)45228-9
Nandi A Yan, L J Jana, C K, Das N (2019) Role of Catalase in Oxidative Stress- and Age-Associated Degenerative Diseases. Oxidative medicine and cellular longevity 9613090. DOI: 10.1155/2019/9613090. DOI: https://doi.org/10.1155/2019/9613090
Oboh G Busari, G M Ademosun, A O, Oyeleye S I (2021) Effect of dietary inclusion of Fireweed (Crassocephalum crepidioides) on behavioral patterns, memory indices, and activities of cholinergic and monoaminergic enzymes in a fruit fly (Drosophila melanogaster) model of Alzheimer's disease. Food Frontiers 3: 206-207. DOI: https://doi.org/10.1002/fft2.121
Ola-Mudathir F K, Maduagwu E N (2014) Antioxidant Effects of Methanol Extract of Allium cepa Linn on cyanide-induced Renal Toxicity in Male Wistar Rats. Niger. J Physiol Sci 29:147-151.
Omoregie E S, Okugbo O T, Oikeh E, Irabor S(2015) Hepatoprotective effect of leaf extracts of Crassocephalum rubens (Juss ex Jacq) S Moore in rifampicin-induced oxidative stress in Swiss mice. Journal of Pharmacy and Bioresources 12(2): 112-119. DOI: https://doi.org/10.4314/jpb.v12i2.5
Orji B O, Obi F O, Modo E U, Osibemhe M, Otitolaiye C A (2020) Amelioration of paracetamol-induced nephrotoxicity in mice by aqueous extract from the calyx of Hibiscus sabdariffa Linn. Biokemistri 32 (1): 23-34.
Pathan M M, M A K Moregaonkar S D, Somkuwar, A P, Gaikwad N Z(2013) Amelioration of paracetamol-induced nephrotoxicity by maytenus emarginata in male Wistar rats. Int J Pharm Pharm Sci 5 (4):471-474.
Perazella M A (2018) Pharmacology behind Common Drug Nephrotoxicities. Clinical Journal of the American Society of Nephrology 13 (12):1897-1908. DOI: https://doi.org/10.2215/CJN.00150118
Salazar H (2014) Overview of Urea and Creatinine. Laboratory Medicine 45 (1): e19–e20. DOI: https://doi.org/10.1309/LM920SBNZPJRJGUT
Sinha A K (1971) Colorimetric assay of catalase. Anal. Biochem 47: 389-394. DOI: https://doi.org/10.1016/0003-2697(72)90132-7
Tripathi S, S Singh S, Garg G, et al. (2019) Metformin ameliorates acetaminophen-induced sub-acute toxicity via antioxidant property”. Drug and chemical toxicology 45 (1):1-9. DOI: https://doi.org/10.1080/01480545.2019.1658769
Ucar F, Taslipinar M Y, Alp B F (2013) The effects of N-acetylcysteine and ozone therapy on oxidative stress and inflammation in acetaminophen-induced nephrotoxicity model. Ren. Fail 35: 640–647. DOI: https://doi.org/10.3109/0886022X.2013.780530
Younus H (2018) Therapeutic potentials of superoxide dismutase. International journal of health sciences 12 (3):88–93.
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