Analysis of Cell Motility with a Successive Sequence of Images

Authors

  • Katarina Zlatolas
  • Peter Veranič
  • Kristijan Jezernik

DOI:

https://doi.org/10.14720/abs.48.1.13535

Keywords:

Cell motility, digital camera, successive sequence of images, software Adobe Illustrator 9.0

Abstract

Analysis of cell motility usually requires several hours of continuousvideo recording of a single view field. Consequently, the method is very much timeconsuming and only a small number of cells is usually included into analysis. To increase the number of cells monitored in an experiment we developed a simpleand expeditious method for analysing the cell motility. A combination of successive series of images of individual cells relocated on CELLocate coverslips andvector graphic editing software Adobe Illustrator 9.0 enabled simultaneous monitoring of locomotion for up to ten times more cells as with previous methods.

Metrics

Metrics Loading ...

Downloads

Download data is not yet available.

References

BONNETON C., J.B. SIBARITA & J.P. THIERY 1999: Relationship between cell migration and cell cycle during the initiation of epithelial to fibroblastoid transition. Cell Motility and the Cytoskeleton 43: 288-295. DOI: https://doi.org/10.1002/(SICI)1097-0169(1999)43:4<288::AID-CM2>3.0.CO;2-Y

BRAY D. 1992: Cell movements. Garland publishing, INC., New York & London, pp. 17-45.

HAY E.D. 1995: An overview of epithelio-mesenchymal transformation. Acta Anat. 154: 8-20. DOI: https://doi.org/10.1159/000147748

MORTON D.M. & R. TCHAO 1994: Regulation of motility and cytoskeletal organization of rat bladder carcinoma cells by cyclic AMP. Cell Motility and the Cytoskeleton 29: 375-382. DOI: https://doi.org/10.1002/cm.970290410

WICK N., S. THURNER, K. PAIHA, R. SEDIVY, I. VIETOR & L.A. HUBER 2003: Quantitative measurement of cell migration using time-lapse videomicroscopy and non-linear system analysis. Springer-Verlag: Histochemistry and Cell Biology, pp. 1-12. DOI: https://doi.org/10.1007/s00418-002-0491-2

Downloads

Published

01.07.2005

Issue

Section

Original Research Paper

How to Cite

Zlatolas, K., Veranič, P., & Jezernik, K. (2005). Analysis of Cell Motility with a Successive Sequence of Images. Acta Biologica Slovenica, 48(1), 13-20. https://doi.org/10.14720/abs.48.1.13535