Vpliv simptomatske bakterijske okužbe na izražanje genov, povezanih z imunostjo v hemocitih kopenskega raka Porcellio scaber

Andraž Dolar; Jernej Ogorevc; Anita Jemec Kokalj

doi:10.14720/abs.66.2.14428

Authors

Andraž Dolar University of Ljubljana, Biotechnical Faculty, Dept. of Biology
Jernej Ogorevc University of Ljubljana, Biotechnical Faculty, Dept. of Animal Science
Anita Jemec Kokalj University of Ljubljana, Biotechnical Faculty, Dept. of Biology

DOI:

https://doi.org/10.14720/abs.66.2.14428

Keywords:

Slovenia

Abstract

Terrestrial isopods Porcellio scaber are exposed to many pathogens and parasites in their natural environment, which can cause tissue damage in the host and affect the immunocompetence and fitness of the organism. Bacterial infection leads to activation of innate immunity mechanisms, such as phagocytosis, formation of reactive oxygen and nitrogen species, activity of antioxidant enzymes, nodule formation, and the process of melanization. Molecular patterns of pathogens or microbes and pathogenesis-induced injury in the host trigger the transcription of genes in haemolymph cells, i.e., haemocytes, which have an important function as mediators of the immune response. In the current study, we examined changes in gene expression during symptomatic bacterial infection with Rhabochlamydia porcellionis and compared them with asymptomatic or healthy P. scaber. We isolated total RNA from the haemolymph (haemocytes) of asymptomatic and symptomatic animals, transcribed it into cDNA, and determined the relative expression of selected immune-related genes (Toll4, Dscam, MyD88, Ppae2a, Cat, MnSod, CypG, A2m, Atg5, and Nos). We found characteristic changes in the expression of selected genes confirming their role in the immune response of P. scaber in case of bacterial infection, and, in addition, biochemical methods showed increased activity of the enzyme alpha-2-macroglobulin and a borderline characteristic increase in the enzyme catalase. Based on the results, we can conclude that the studied genes represent molecular markers of immune response that can be used in various environmental studies.

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References

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