The RepFIIA replicon of the natural Escherichia coli plasmid pRK100

Authors

  • Marjanca Starčič Erjavec
  • Darja Žgur-Bertok

DOI:

https://doi.org/10.14720/abs.49.2.15068

Keywords:

plasmid, RepFIIA replicon, Enterobacteriaceae, nucleotide divergence

Abstract

The aim of the presented study was to identify the similarity of the plasmid pRK100 RepFIIA replicon (replication region) with similar replicons of other known plasmids of Enterobacteriaceae. For this purpose, within the determined nucleotide sequence of pRK100, theRepFIIA replicon rep genes/regions were identified. The nucleotide sequences of the pRK100 determined rep genes/regions were subsequently compared with the nucleotide sequences of other RepFIIA replicon rep genes/regions deposited in GenBank. Further, the nucleotide divergence between them was calculated. The obtained results clearly demonstrated, that the individual pRK100 rep regions are the same/most similar to rep regions from different plasmids. RepA2 of pRK100 is most similar to repA2 of pCP301, pINV_F6_M1382, pWR501 and R1, copA is the same as copA of plasmids pC15-1a and R100, repA6 of pRK100 is the same as repA6 in plasmids pC15-1a, pCP301, pINV_F6_M1382, pWR501, R1 and R100, repA1 is most similar to repA1 of the plasmid p1658/79, and repA4 of pRK100 is most similar to repA4 of pC15-1a. Hence, the composition of the pRK100 RepFIIA replicon is mosaic and unique among the plasmids.

References

ALTSCHUL, S. F., T. L. MADDEN, A. A. SCHÄFFER, J. ZHANG, Z. ZHANG, W. MILLER, & D. J. LIPMAN 1997: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389–3402. DOI: https://doi.org/10.1093/nar/25.17.3389

AMBROŽIČ J., A. OSTROVERŠNIK, M. STARČIČ, I. KUHAR, M. GRABNAR & D. ŽGUR-BERTOK 1998: Escherichia coli ColV plasmid pRK100: genetic organization, stability and conjugal transfer. Microbiology 144: 343–352. DOI: https://doi.org/10.1099/00221287-144-2-343

BLOMBERG P., K. NORDSTROM & E. G. WAGNER 1992: Replication control of plasmid R1: RepA syn- thesis is regulated by CopA RNA through inhibition of leader peptide translation. EMBO J. 11: 2675–2683. DOI: https://doi.org/10.1002/j.1460-2075.1992.tb05333.x

BOYD, E. F., C. W. HILL, S. M. RICH & D. L. HARTL 1996: Mosaic structure of plasmids from natural populations of Escherichia coli. Genetics 143:1091–100. DOI: https://doi.org/10.1093/genetics/143.3.1091

COUTURIER, M., F. BEX, P. L. BERGQUIST, & W. K. MAAS 1988: Identification and classification of bacte- rial plasmids. Microbiol. Rev. 52: 375–395. DOI: https://doi.org/10.1128/mr.52.3.375-395.1988

FELSENSTEIN, J. 1993: PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author.

Department of Genetics, University of Washington, Seattle.

FELSENSTEIN, J. 1989: PHYLIP – Phylogeny Inference Package (Version 3.2) Cladistics 5:164–166.

FIRTH, N., K. IPPEN-IHLER, & R. A. SKURRAY 1996: Structure and function of the F factor and mechanism of conjugation, p. 2377–2401. In F. C. Neidhardt, et al. (editors), Escherichia coli and Salmonella, 2nd edition. ASM Press, Washington.

HELINSKI, D. R., A. E. TOUKDARIAN, & R. P. NOVICK 1996: Replication control and other stable main- tenance mechanisms of plasmids, p. 2295–2324. In F. C. Neidhardt, et al. (editors), Escherichia coli and Salmonella, 2nd edition. ASM Press, Washington.

HOLMES, M. L., F. PFEIFER, & M. L. DYALL-SMITH 1995: Analysis of the halobacterial plasmid pHK2 minimal replicon. Gene 153:117–121. DOI: https://doi.org/10.1016/0378-1119(94)00761-G

JIANG, T., Y.-N. MIN, W. LIU, D. D. WOMBLE, & R. H. ROWND 1993: Insertion and deletion muta- tions in the repA4 region of the IncFII plasmid NR1 cause unstable inheritance. J. Bacteriol. 175:5350–5358. DOI: https://doi.org/10.1128/jb.175.17.5350-5358.1993

KADO, C. I. 1998: Origin and evolution of plasmids. Antoine van Leeuwenhoek 73:117–126. DOI: https://doi.org/10.1023/A:1000652513822

OSBORN, A. M., F. M. DA SILVA TATLEY, L. M. STEYN, R. W. PICKUP, & J. R. SAUNDERS 2000: Mosaic plasmids and mosaic replicons: evolutionary lessons form the analysis of genetic diversity in IncFII-related replicons. Microbiology 146: 2267–2275. DOI: https://doi.org/10.1099/00221287-146-9-2267

SOLAR, G. DEL, R. GIRALDO, M. J. RUIZ-ECHEVARRIA, M. ESPINOSA, & R. DIAZ-OREJAS 1998: Replication and control of circular bacterial plasmids. Microbiol. Mol. Biol. Rev. 62:434–464. DOI: https://doi.org/10.1128/MMBR.62.2.434-464.1998

STARČIČ ERJAVEC, M., W. GAASTRA, D. ŽGUR-BERTOK 2003: tra region of the natural conjugative Es- cherichia coli plasmid pRK100 is F-like. Acta biol Slovenica 45:9–15.

STARČIČ ERJAVEC, M. 2003: Mosaic structure and regulation of conjugal transfer of the Escherichia coli plasmid pRK100. PhD thesis, University. of Utrecht, The Netherlands.

THOMPSON, J. D., D. G. HIGGINS & T. J. GIBSON 1994: CLUSTAL W: improving the sensitivity of pro- gressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673–4680. DOI: https://doi.org/10.1093/nar/22.22.4673

VANOOTEGHEM, J.-C. & G. R. CORNELIS 1990: Structural and functional similarities between the replication region of the Yersinia virulence plasmid and the RepFIIA replicons. J. Bacteriol. 172:3600–3608. DOI: https://doi.org/10.1128/jb.172.7.3600-3608.1990

ZILLIG, W., H. P. ARNOLD, I. HOLZ, D. PRANGISHVILI, A. SCHWEIER, K. STEDMAN, Q. SHE, H. PHAN, R. GARRETT, & J. K. KRISTJANSSON 1998: Genetic elements in the extremely thermophilic archaeon Sulfolobus. Extremophiles 2:131–140. DOI: https://doi.org/10.1007/s007920050052

ŽGUR-BERTOK D, E. MODRIČ & M. GRABNAR 1990: Aerobactin uptake system, ColV production, and drug resistance encoded by a plasmid from an urinary tract infection Escherichia coli strain of human origin. Can. J. Microbiol. 36:297–299. DOI: https://doi.org/10.1139/m90-051

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Published

01.12.2006

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Original Research Paper

How to Cite

Starčič Erjavec, M., & Žgur-Bertok, D. (2006). The RepFIIA replicon of the natural Escherichia coli plasmid pRK100. Acta Biologica Slovenica, 49(2). https://doi.org/10.14720/abs.49.2.15068

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