Using carbon fibre microelectrodes to monitor the oxidative metabolism of blowfly eyes

Authors

  • Andrej Meglič
  • Gregor Belušič
  • Gregor Zupančič

DOI:

https://doi.org/10.14720/abs.52.1.15175

Keywords:

blowfly eye, Calliphora vicina – chalky, carbon fibre electrode, PO2 measurement, amperometry

Abstract

The oxidative metabolism in animal tissues can be conveniently monitored by measuring tissue PO2 with a carbon fibre microelectrode. We have established a recording configuration in a living animal by insertion of a carbon fibre electrode (CFE) into the retina of a blowfly (Calliphora vicina – chalky). The current flowing over an exposed carbon disc at the tip of an insulated carbon fibre with 5 µm diameter is linearly proportional to PO2 when the PO2 was varied between 0 kPa (100% N2) and 100 kPa (100% O2) in the recording chamber. The slight changes in sensitivity of CFE during the recording time were corrected by calibrations
performed at the start and at the end of the experiments. Exposure of the eye to bright light caused a drop in tissue PO2. Hypoxia increased with the stimulation time, reaching a maximum after about 20 s (∆PO2=11.6 kPa). These results are in good agreement with direct measurements of O2 consumption in isolated eyes.

References

Chow R.H. & L. von Rüden 1995: In Single Channel Recording, 2nd ed. Plenum Press City .pp 245–275. DOI: https://doi.org/10.1007/978-1-4419-1229-9_11

Hamdorf K., P . Hochstrate, G. Höglund , B. Burbach & U. Wiegand 1988: Light activation of the sodium pump in blowfly photoreceptors. J Comp Physiol. A 162:285–300. DOI: https://doi.org/10.1007/BF00606117

Mojet M.H., J. Tinbergen & D.G. Stavenga 1991: Receptor potential and light induced mitochondrial activation in blowfly photoreceptor mutants. J Comp Physiol A. 168:305–312. DOI: https://doi.org/10.1007/BF00198350

Mojet M.H., E. Mills & M.R. Duchen 1997: Hypoxia-induced catecholamine secretion in isolated newborn rat adrenal chromaffin cells is mimicked by inhibition of mitochondrial respiration. J Physiol. 504: 175–189. DOI: https://doi.org/10.1111/j.1469-7793.1997.175bf.x

Pangršič T., P. Stušek , G. Belušič & G. Zupančič 2005: Light dependence of oxygen consumption by blowfly eyes recorded with a magnetic diver balance. J Comp Physiol A. 191: 75–84. DOI: https://doi.org/10.1007/s00359-004-0571-y

Poitry S., M. Tsacopulos, A. Fein & M.C. cornwall 1996: Kinetics of oxygen consumption and lightinduced changes of nucleotides in solitary rod photoreceptors. J Comp Physiol A. 108: 75–87. DOI: https://doi.org/10.1085/jgp.108.2.75

Poitry S. & M. Widmer 1996: Kinetics of oxygen consumption after a flash of light in the lateral ocellus of the barnacle. Biophys J. 54: 655–667. DOI: https://doi.org/10.1016/S0006-3495(88)83001-7

Schulte A. & R.H. Chow 1996: A simple method for insulating carbon-fiber microelectrodes using anodic electrophoretic deposition of paint. Anal. Chem. 68: 3054–3058. DOI: https://doi.org/10.1021/ac960210n

Smits R.P., N.M. Jansonius & D.G. Stavenga 1995: Dependence of receptor potential and redox state of mitochondrial cytochromes on oxygen fraction measured in the blowfly eye in vivo. J. Comp. Physiol. A 177: 105–110. DOI: https://doi.org/10.1007/BF00243402

Stavenga D.G. & J. Tinbergen 1983: Light dependence of oxidative metabolism in fly compound eyes studied in vivo by microspectrofluorimetry. Naturwissenschaften 70: 618–620. DOI: https://doi.org/10.1007/BF00377410

Stavenga, D.G. 1995: Insect retinal pigments: spectral characteristics and physiological functions. Prog. Retin. Eye Res. 15: 231–259. DOI: https://doi.org/10.1016/1350-9462(95)00011-9

Tinbergen J. & D.G. Stavenga 1986: Photoreceptor redox state monitored in vivo by transmission and fluorescence microspectrophotometry in blowfly compound eyes. Vision Res. 26: 239–243. DOI: https://doi.org/10.1016/0042-6989(86)90018-0

Tinbergen J. & D.G. Stavenga 1987: Spectral sensitivity of light induced respiratory activity of photoreceptor mitochondria in the intact fly. J Comp Physiol A. 160: 195–203. DOI: https://doi.org/10.1007/BF00609726

Tsacopoulos M. & S. Poitry 1982: Kinetics of oxygen consumption after a single flash of light in photoreceptors of the drone (Apis mellifera). J. Gen. Physiol. 80: 19–55. DOI: https://doi.org/10.1085/jgp.80.1.19

Widmer H., S. Poitry & M. Tsacopoulos 1990: The increase of oxygen consumption after a flash of light is tightly coupled to sodium pumping in the lateral ocellus of barnacle. J. Gen. Physiol. 96: 83–108. DOI: https://doi.org/10.1085/jgp.96.1.83

Zupančič G. 2003: A method for dynamic spectrophotometric measurements in vivo using principal component analysis-based spectral deconvolution. Pflüg Arch Eur J Physiol. 447: 109–119. DOI: https://doi.org/10.1007/s00424-003-1147-3

Downloads

Published

01.07.2009

Issue

Section

Original Research Paper

How to Cite

Meglič, A., Belušič, G., & Zupančič, G. (2009). Using carbon fibre microelectrodes to monitor the oxidative metabolism of blowfly eyes. Acta Biologica Slovenica, 52(1), 19-28. https://doi.org/10.14720/abs.52.1.15175

Similar Articles

1-10 of 23

You may also start an advanced similarity search for this article.