Toxicity of the organophosphorous insecticide chlormephos to the earthworm Eisenia andrei and the terrestrial isopod Porcellio scaber


  • Rok Hrženjak
  • Primož Zidar
  • Tina Virant Celestina
  • Nevenka Kožuh Eržen
  • Lucija Kolar



ecotoxicity, organophosphates, chlormephos, soil exposure, soil invertebrates


In the present study we determined the toxicity of chlormephos to two common soil organisms – earthworms (Eisenia andrei) and isopods(Porcellio scaber) using Lufa 2.2 soil. The LC50 values for the effects on earthworm and isopod survival were 58 and 76 mg/kg dry soil, respectively. Mass change of earthworms and behaviour of isopods were more sensitive end points as survival. Based on earthworm body mass changes, NOEC and LOEC values were 1 and 3 mg/kg dry soil, respectively. The earthworms did not reproduce even at the lowest chlormephos concentration (LOEC < 1 mg/kg dry soil). Isopods significantly avoided burrowing in soil treated with ≥40 mg/kg dry soil. Compared with some other organophosphate insecticides, chlormephos was highly toxic to beneficial terrestrial invertebrates.


Barry M.J., 1999: The effects of a pesticide on inducible phenotypic plasticity in Daphnia. Environ. Pollut. 104: 217–224. DOI:

Bayley M. 1995: Prolonged effects on the insecticide dimethoate on locomotor behaviour in the woodlouse Porcellio Scaber, Latr. (Isopoda). Ecotoxicology 4: 79–90. DOI:

Drobne D., Blažič M., Van Gestel C.A.M., Lešer V., Zidar P., Jemec A. & Trebše P. 2008: Toxicity of imidacloprid to the terrestrial isopod Porcellio scaber (Isopoda, Crustacea). Chemosphere 71: 1326–1334. DOI:

Dutta H.M. & Maxwell L.B. 2003: Histological examination of sublethal effects of diazinon on ovary of bluegill, Lepomis macrochirus. Environ. Pollut. 121: 95–102. DOI:

Engenheiro E.L., Hankard P.K., Sousa J.P., Lemos M.F., Weeks J.M. & Soares A M.V.M. 2005: Influence of dimethoate in acetylcholinesterase activity and locomotor function in terrestrial isopods. Environ. Toxicol. Chem. 24: 603–609. DOI:

EU 2008: Commission Regulation (EC) No 689/2008 of the European Parliament and of the Council of 17 June 2008 concerning the export and import of dangerous chemicals. Brussels, EU, 204: 1–35. <

PDF>, (cited: 10. 12. 2009).

EU 2006: Commission Regulation (EC) No 777/2006 of 23 May 2006 amending Annex I to Regulation (EC) No 304/2003 of the European Parliament and of the Council concerning the export and import of dangerous chemicals. Brussels, EU, 136: 9–17. <>, cited: 10. 12. 2009).

Fischer E., Farkos S., Hornung E. & Past T. 1997: Sublethal effects of an organophosphorus insecticide, dimethoate, on the isopod Porcellio scaber Latr. Comp. Biochem. Physiol. 116C: 161–166. DOI:

FLO 2007: Generic Fairtrade Standards; Fairtrade Labelling Organizations International, FLO Prohibited Material List. Bonn: 1–9.<>, (cited: 10. 12. 2009).

Footprint 2006: The FOOTPRINT Pesticide Properties DataBase. Database collated by the University of Hertfordshire as part of the EU-funded FOOTPRINT project (FP6-SSP-022704), <>, (cited: 10. 12. 2009).

Fulton M.H. & Key P.B. 2001: Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects. Environ. Toxicol. Chem. 20: 37–45. DOI:

Gully D.D., Boelter A.M. & Bergman H.L. 1991: TOXSTAT® ver. 3.3. University, Fish physiology and toxicology laboratory, Department of zoology and physiology, Wyoming, computer program.

Gupta R.C. 2007: Organophosphates and carbamates. In: Gupta R.C. (ed.): Veterinary toxicology, Academic Press, Elsevier, London, New York, pp. 477–488. DOI:

Haanstra L., Doelman P. & Oude Voshaar J.H. 1985: The use of sigmoidal dose response curves in soil ecotoxicological research. Plant Soil 84: 293–297. DOI:

Hamilton M.A., Russo R.C. & Thurston R.V. 1978: Trimmed Spearman-Karber method for estimating lethal concentrations in toxicity bioassays. Environ. Sci. Technol. 12: 417. DOI:

Hanazato T. 1998: Response of a zooplankton community to insecticide application in experimental ponds: a review and the implications of the effects of chemicals on the structure and functioning of freshwater communities. Environ. Pollut. 101: 361–373. DOI:

Hornung E., Farkas S. & Fischer E 1998: Tests on the isopod Porcellio scaber. In: Løkke H. & Van Gestel C.A.M. (eds.): Handbook of soil invertebrate toxicity tests. Wiley and Sons, Chichester, pp. 207–226.

Lanno R.P., Stephenson G.L. & Wren C.D. 1997: Applications of toxicity curves in assessing the toxicity of diazinon and pentachlorophenol to Lumbricus terrestris in natural soils. Soil Biol. Biochem. 29: 689–692. DOI:

Loureiro S., Soares A.M. & Nogueira A.J. 2005: Terrestrial avoidance behaviour tests as screening tool to assess soil contamination. Environ. Pollut. 138: 121–131. DOI:

Lynch V.P. 1978: Chlormephos. In: Zweig G. & Sherman J. (ed.): Analytical methods for pesticides and plant growth regulators, 1st ed. Academic Press, NY, pp. 49–55. DOI:

Mosleh Y.Y., Paris-Palacios S., Couderchet M. & Vernet G. 2003: Acute and sublethal effects of two insecticides on earthworms (Lumbricus terrestris L.) under laboratory conditions. Environ. Toxicol. 18: 1–8. DOI:

OECD 2004: Earthworm reproduction test (Eisenia fetida/Eisenia andrei). Guideline for the testing of chemicals No. 222. In: Organization for Economic Cooperation and Development. Paris, pp. 1–18.

Olvera-Velona A., Capowiez Y., Mascle O., Ortiz-Hernandez L. & Benoit P. 2008: Assessment of the toxicity of ethyl-parathion to earthworms (Aporrectodea caliginosa) using behavioural, physicological and biochemical markers. Appl. Soil Ecol. 40: 476–483. DOI:

Panda S. & Sahu S.K. 2000: Assesment of recovery of population, biomass and reproduction of the DOI:

earthworm Drowida willsi followinf the application of malathion under field conditions. Biol.

Fertil. Soils 32: 82–88.

Pantani C., Pannunzio G., De Cristofaro M., Novelli A.A. & Salvatori M. 1997: Comparative acute toxicity of some pesticides, metals, and surfactants to Gammarus italicus Goedm. and Echinogammarus tibaldii pink and stock (Crustacea: Amphipoda). Bull. Environ. Contam. Toxicol. 59: 963–967. DOI:

Qi-xing Z., Qian-ru Z. & Ji-dong L. 2006: Toxic effects of acetochlor and methamidophos on earthworm Eisenia fetida in phaiozem, northeast China. J. Environ. Sci. 18: 741–745.

Sandahl J.F., Baldwin D.H., Jenkins J.J. & Scholz N.L. 2005: Comparative thresholds for acetylcholinesterase inhibition and behavioral impairment in coho salmon exposed to chlorpyrifos. Environ. Toxicol. Chem. 24: 136–145 DOI:

Sherrarda R.M., Bearrb J.S., Murray-Guldec C.L., Rodgers j.r. J.H. & Shahd Y.T. 2004: Feasibility of constructed wetlands for removing chlorothalonil and chlorpyrifos from aqueous mixtures. Environ. Pollut. 127: 385–394. DOI:

Venkateswara Rao J., Surya Pavan Y. & Madhavendra S.S. 2003: Toxic effects of chlorpyrifos on morphology and acetylcholinesterase activity in the earthworm, Eisenia foetida. Ecotoxicol. Environ. Saf. 54: 296–301. DOI:

Vial A. 1991: Report on the acute toxicity test of C177 technical to earthworm (Eisenia eisenia foetida). Ciba-Geigy Ltd, Product safety, Ecotoxicology, Basel, Switzerland. Test No. 918079. ECRPsubmission, unpublished report.

Vink K., Dewi L., Bedaux J., Tompot A., Hermans M. & Straalen N.M.V. 1995: The importanceof the exposure route when testing the toxicity of pesticides to saprotrophic isopods. Environ.Toxical. Chem. 14: 1225–1232. DOI:

Weyman G.S. 1997: 14-day acute toxicity study with chlorfenvinphos in the earthworm (Eisenia foetida). Covance Laboratory, North Yorkshire, England. Laboratory project ID 628/87 and 954–96-314 (American Cyanamid company), report No. ECO 96–314. American Cyanamid company,Princeton, New Jersey, Unpublished.

Yasmin S., D'Souza D. 2007: Effect of pesticides on the reproductive output of Eisenia fetida. Bull. Environ. Contam. Toxicol. 79: 529–532. DOI:

Zidar P., Božič J. & Štrus J. 2005: Behavioural response in the terrestrial isopod Porcellio scaber (Crustacea) offered a choice of uncontaminated and cadmium contaminated food. Ectoxicology 14: 493–502. DOI:

Zidar P., Drobne D., Štrus J., Van Gestel C.A.M. & Donker M. 2004: Food selection as a means of Cu intake reduction in the terrestrial isopod Porcellio scaber (Crustacea, Isopoda). Appl. Soil Ecol. 25: 257–265. DOI:






Original Research Paper

How to Cite

Hrženjak, R., Zidar, P., Virant Celestina, T., Kožuh Eržen, N., & Kolar, L. (2009). Toxicity of the organophosphorous insecticide chlormephos to the earthworm Eisenia andrei and the terrestrial isopod Porcellio scaber. Acta Biologica Slovenica, 52(2), 85-94.

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