Genetic background of uropathogenic Escherichia coli isolates from Slovenia in relation to fluoroquinolone and sulfamethoxazole/trimethoprim resistance
DOI:
https://doi.org/10.14720/abs.54.2.15475Keywords:
Escherichia coli, urinary tract, phylogenetic groups, virulence trait, fluoroquinolone resistance, sulfamethoxazole/trimethoprim resistanceAbstract
A total of 99 E. coli urinary tract isolates were investigated for phylogenetic groups and 21 virulence related genes in relation to fluoroquinolone and sulfamethoxazole/trimethoprim resistance. We found that the B2 group was by far the most prevalent among susceptible isolates, while resistant isolates were more evenly distributed among groups A, B2 and D. Isolates from the B2 group exhibited the highest prevalence of virulence factors. Virulence genes hlyA, iroN and kpsMTII were statistically associated with fluoroquinolone susceptible isolates and picU with sulfamethoxazole/trimethoprim susceptible isolates. Fluoroquinolone susceptible
isolates of the phylogenetic group A were significantly associated with genes papGII, kpsMTII and iss and the susceptible group B2 isolates with genes hra in iroN. Among isolates susceptible to sulfamethoxazole/trimethoprim the presence of the hra gene
was statistically significantly associated with phylogenetic group B2, while among resistant isolates, papGII was associated with phylogenetic group D.
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Car, J., Švab, I., Kersnik, J., Vegnuti, M,. 2003. Management of lower urinary tract infection in women by Slovene GPs. Fam. Pract., 20, 452–6. DOI: https://doi.org/10.1093/fampra/cmg421
Clermont, O., Bonacorsi. S., Bingen, E., 2000. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl. Environ. Microbiol., 66, 4555–4558. DOI: https://doi.org/10.1128/AEM.66.10.4555-4558.2000
Clinical and Laboratory Standards Institute, 2007. Performance standards for antimicrobial susceptibility testing; seventeenth informational supplement. M100-S17. Clinical and Laboratory Standards Institute, Wayne, PA.
Drews, S.J., Poutanen, S.M., Mazzulli, T., McGeer A.J., Sarabia, A., Pong-Porter, S., Rzayev, Y., Willey, B., Green, K., Low, D.E., 2005. Decreased prevalence of virulence factors among ciprofloxacinresistant uropathogenic Escherichia coli isolates. J. Clin. Microbiol., 43, 4218–20. DOI: https://doi.org/10.1128/JCM.43.8.4218-4220.2005
Horcajada, J.P., Soto, S., Gajewski, A., Smithson, A., Jiménez de Anta, M.T., Mensa, J., Vila, J., Johnson, J.R., 2005. Quinolone-resistant uropathogenic Escherichia coli strains from phylogenetic group B2 have fewer virulence factors than their susceptible counterparts. J. Clin. Microbiol.; 43: 2962–4. DOI: https://doi.org/10.1128/JCM.43.6.2962-2964.2005
Johnson, J.R., Kuskowski, M.A., O’Bryan, T.T., Colodner, R., Raz, R. 2005. Virulence genotype and phylogenetic origin in relation to antibiotic resistance profile among Escherichia coli urine sample isolates from Israeli women with acute uncomplicated cystitis. Antimicrob. Agents. Chemother., 49, 26–31. DOI: https://doi.org/10.1128/AAC.49.1.26-31.2005
Johnson, J.R., Kuskowski, M.A., Owens, K., Clabots, C., Singer, R.S., 2009. Virulence genotypes and phylogenetic background of fluoroquinolone-resistant and susceptible Escherichia coli urine isolates from dogs with urinary tract infection. Vet. Microbiol., 136, 108–114. DOI: https://doi.org/10.1016/j.vetmic.2008.10.006
Johnson, J.R., Kuskowski, M.A., Owens, K., Gajewski, A., Winokur, P.L., 2003. Phylogenetic origin and virulence genotype in relation to resistance to fluoroquinolones and/or extended-spectrum cephalosporins and cephamycins among Escherichia coli isolates from animals and humans. J. DOI: https://doi.org/10.1086/377455
Infect. Dis., 188, 759–68.
Le Bouguenec, C., Archambaud, M., Labigne, A., 1992. Rapid and specific detection of the pap, afa, and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction. J. Clin. Microbiol., 30, 1189–1193. DOI: https://doi.org/10.1128/jcm.30.5.1189-1193.1992
Lindič, J., 2005. Pristop k bolniku z okužbo sečil. [Accession to a patient with urinary tract infection]. In: Fras Z, Poredoš P, eds. 47. Tavčarjevi dnevi. [47th Tavčar’s days]. Zbornik prispevkov. [Proceedings]. Ljubljana: Medical Faculty;. p. 137–48.
Moreno, E., Prats, G., Sabaté, M., Pérez, T., Johnson, J., Andreu, A., 2006. Quinolone, fluoroquinolone and trimethoprim/sulfamethoxazole resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherichia coli. J. Antimicrob. Chemother., 57, 204–211. DOI: https://doi.org/10.1093/jac/dki468
Oelschlaeger, T.A., Dobrindt, U., Hacker, J., 2002.Virulence factors of uropathogens. Curr. Opin. Urol., 12, 33–8. DOI: https://doi.org/10.1097/00042307-200201000-00007
Piatti, G., Mannini, A., Balistreri, M., Schito, A.M., 2008. Virulence factors in urinary Escherichia coli strains: Phylogenetic background and quinolone and fluoroquinolone resistance. J. Clin. Microbiol., 46, 480–487. DOI: https://doi.org/10.1128/JCM.01488-07
Picard, B., Garcia, J.S., Gouriou, S., Duriez, P., Brahimi, N., Bingen, E., Elion, J., Denamur, E., 1999. The link between phylogeny and virulence in Escherichia coli extraintestinal infection. Infect. Immun., 67, 546–53. DOI: https://doi.org/10.1128/IAI.67.2.546-553.1999
Takahashi, A., Muratani, T., Yasuda, M., Takahashi, S., Monden, K., Ishikawa, K., Ishikawa, K., Kiyota, H., Arakawa, S., Matsumoto, T., Shima, H., Kurazono, H., Yamamoto, S., 2009. Genetic profiles of fluoroquinolone-resistant Escherichia coli isolates obtained from patients with cystitis: Phylogeny, virulence factors, paiusp subtypes, and mutation patterns. J. Clin. Microbiol.,47, 791–5. DOI: https://doi.org/10.1128/JCM.01740-08
Vila, J., Simon, K., Ruiz, J., Horcajada, J.P., Velasco, M., Barranco, M., Mensa, J., 2002. Are quinoloneresistant uropathogenic Escherichia coli less virulent? J. Infect. Dis., 186, 1039–42. DOI: https://doi.org/10.1086/342955
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