In vitro propagation of Lilium martagon L. var. cattaniae Vis. and evaluation of genotoxic potential of its leaves and bulbs extracts


  • Una Glamočlija
  • Sanin Haverić
  • Jasmina Čakar
  • Anisa Rahamović
  • Damir Marjanović



in vitro culture, ultrasound assisted extraction, micronuclei, genotoxic, Lilium martagon L. var. cattaniae Vis.


Lilium martagon L. var. cattaniae Vis. (Liliaceae) is endemic plant of Dinaridi mountain. In this work we established protocol for fast in vitro propagation and multiplication of Lilium martagon var. cattaniae. The aim was to enable fast production of plant material as potential source of pharmaceutically valuable secondary metabolites. Seeds of L.martagon var. cattaniae were germinated on a Murashige and Skoog basal medium with a supplement of 0.15 mg/l gibberellic acid (GA3), and multiplication was performed on MS medium supplemented with 0.1 mg/l gibberellic acid (GA3), 0.2 mg/l indole-3-butyric acid (IBA) and 0.5 mg/l 6-ben- zylaminopurine (BAP). We used ultrasound assisted extraction to prepare extracts of leaves and bulbs of Lilium martagon var. cattaniae, which were evaluated for their genotoxic potential using Allium test and cytokinesis-block micronucleus test in human lymphocytes culture. There was statistically significant difference between all used concentrations of lilium extracts and control on proliferation of cells of root tip of onion (Allium cepa). In cytokinesis-block micronucleus test no statistically significant difference between frequencies of analyzed parameters in samples treated with tested concentrations of extracts and control was obtained.


Chendke, P.K., Fogler, H.S., 1975. Macrosonics in industry: 4. Chemical processing. Ultrasonics, 13(1), 31-37. DOI:

Dolatowski, Z.J., Stadnik, J., Stasiak, D., 2007. Applications of ultrasound in food technology. Acta Sci. Pol. Technol. Aliment., 6(3), 89-99.

Eastmond, D.A., Tucker, J.D., 1989. Identification of aneuplody-inducing agents using cytokinesis- blocked human lymphocytes and an antikinetochore antibody. Environ. Mol. Mutagen., 13, 34-43. DOI:

Eisenreichová, E., Haladová, M., Mučaji, P., Grančai, D., 2004. The study of constituents of Lilium candidum L. Acta Facultatis Pharmaceuticae Comenianae, Department of Pharmacognosy and Botany, Faculty of Pharmacy,Comenius University, Bratislava, pp. 11.

Fenech, M., Chang, W.P., Kirsch-Volders, M., Holland, N., Bonassi, S., Zeiger, E., 2003. HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res., 534, 65–75. DOI:

Fenech, M., 2000. The in vitro micronucleus technique. Mutat. Res., 455, 81-95. DOI:

Khare, C.P., 2007. Indian Medicinal Plants: An Illustrated Dictionary. Springer, pp. 374. DOI:

Matthews, V., 1980. Lilium L. In: Tutin T. G., Heywood V.H., Burges N.A., Moore D.M., Valentine D.H., Walters S.M., Webb D.A. (eds.), Flora Europaea, Cambridge University Press., pp. 5-35.

Murashige T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15, 473-479. DOI:

Parić, A., Hindija, J., Muratović, E., Pojskić, N., Bajrović, K., 2008. Breaking dormancy of two en- demic Lilium species: Lilium bosniacum (G. Beck) Beck ex Fritsch and Lilium martagon L. var. cattaniae Vis. Seed science and technology, 36(3), 788-791. DOI:

Pelkonen, V.P., 2005. Biotechnological approaches in lily (Lilium) production, Faculty of Science, Department of Biology, University of Oulu, Finland.

Petrovic, D.M., Jacimovic-Plavšic, M.M. 1992: Aronia melancarpa and propagation in vitro. In: Da- miano, C., Read, P.E., Preece, J.E., Ladyman, J.A.R., Debergh, P. (eds.): ISHS Acta Horticulturae 300: In Vitro Culture, XXIII IHC. Firenze, pp. 133-136. DOI:

Pieroni, A., Dibra, B., Grishaj, G., Grishaj, I., Maçai, S.G., 2005. Traditional phytotherapy of the Albanians of Lepushe, Northern Albanians Alps. Fitoterapia, 76, 379-399. DOI:

Redžić, S., 2010. Wild medicinal plants and their usage in traditional human therapy (Southern Bosnia and Herzegovina, W. Balkan). Journal of Medicinal Plants Research 4(11), 1003-1027.

Remington: The Science and Practice of Pharmacy. 2005: 21st ed. Lippincott Williams & Wilkins, Philadelphia, pp. 985-987.

Rouhani, S., Alizadeh, N., Salimi, S., Haji-Ghasemi, T., 2009. Ultrasonic Assisted Extraction of Natural Pigments from Rhizomes of Curcuma Longa L. J.Prog. Color, Colorants, Coatings. PCCC-J-09-0119.

Šilić, Č. 1990: Endemične biljke. 3rd edition. Svjetlost, Sarajevo.

Weng, Y.M., Chuang, Y.C., Chen, W.L., Tseng, C.Y.. 2004. Ingredient extracting efficiency and fun- ctional properties of Chinese medicinal herbal wines as affected by ultrasound-assisted extraction. 2004 IFT Annual Meeting and Food Expo. Las Vegas, Nevada 114F-20.






Original Research Paper

How to Cite

Glamočlija, U., Haverić , S., Čakar, J., Rahamović, A., & Marjanović, D. (2010). In vitro propagation of Lilium martagon L. var. cattaniae Vis. and evaluation of genotoxic potential of its leaves and bulbs extracts. Acta Biologica Slovenica, 53(2), 53-60.

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