Preliminary multispecies test of a model for non-lethal estimation of metabolic activity in freshwater crayfish

Tatjana Simčič; Franja Pajk; Anton Brancelj; Al Vrezec

doi:10.14720/abs.55.1.15522

Authors

Tatjana Simčič
Franja Pajk
Anton Brancelj
Al Vrezec

DOI:

https://doi.org/10.14720/abs.55.1.15522

Keywords:

electron transport system (ETS) activity, crayfish, size scaling, method

Abstract

We tested the applicability of electron transport system (ETS) derived from a single leg as a tool for non-lethal assessment of metabolicactivity in freshwater crayfish. ETS activity of the whole body and of a leg was measured in four crayfish (Arthropoda, Crustacea, Decapoda)species: two European (Astacus astacus, Austro- potamobius torrentium), and two North American (Orconectes limosus, Pacifastacusleniusculus). Mass scaling of whole body ETS activity (ETS_whole) and leg ETS activity (ETS_leg) was not significantly different for the European A. astacus and the America O. limosus. Therefore common models were constructed and tested on the remaining two species. The ratio ETS_whole/ETS_leg was significantly positively related to body mass. In the first model (model 1) ETS_whole was calculated from ETS_leg multiplied by the ratio estimated from the known body mass. ETS_whole of A. torrentium was underestimated by this model, because they mature at smaller body size than the larger species. A direct relation between ETS_leg and ETS_whole was therefore proposed as a general model (model 2), since they are correlated similarly in the studied species. The results show that model 2 is suitable for estimating the whole body ETS activity from leg ETS activity for the four investigated decapods.

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Table 1: Results of statistical testing (for details see methods) of the differences in intercept and slope between Orconectes limosus and Astacus astacus for the specified relationships (ETSwhole – whole body ETS activity, ETSleg – leg ETS activity, PROTwhole – whole body protein content, PROTleg – protein content of a leg).

Tabela 1: Rezultati statističnega testiranja (za podrobnosti glej metode) razlik v odseku in naklonu med vrstama Orconectes limosus in Astacus astacus za podana razmerja (ETSwhole – aktivnost ETS celega telesa, ETSleg – aktivnost ETS noge, PROTwhole – vsebnost proteinov v celem telesu, PROTleg – vsebnost proteinov v nogi).