Lack of introns in putative parasitism factor gene, expansin (expB2) from pale potato cyst nematode Globodera pallida

Authors

  • Barbara Gerič Stare
  • Saša Širca
  • Gregor Urek

DOI:

https://doi.org/10.14720/abs.55.1.15526

Keywords:

Cell wall degradation, Globodera pallida, effectors, expansin, intron, parasitism factor, plant-parasitic nematode, potato cyst nematode

Abstract

Expansins are a group of plant cell wall loosening proteins. In animals, functional expansin (EXPB1) has been discovered in the golden potato cyst nematode Globodera rostochiensis. In plant-parasitic nematodes expansins act as the parasitism factors or effectors. Molecularvariability of another expansin (expB2) gene was eva- luated in the diverse populations of the G. rostochiensis. Comparison of the expB2 genestructure in the two potato cyst nematode species, G. rostochiensis and G. pallida, revealed lack of all four introns in expB2 gene of G. pallida species. Possible loss of introns in Gp-expB2 is discussed.

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References

Boudet, N., Aubourg, S., Toffano-Nioche, C., Kreis, M., Lecharny, A., 2001. Evolution of intron/ exon structure of DEAD helicase family genes in Arabidopsis, Caenorhabditis, and Drosophila. Genome Research, 11, 2101–2114. DOI: https://doi.org/10.1101/gr.200801

Cosgrove, D.J., 2000. Loosening of plant cell walls by expansins. Nature, 407, 321–326. DOI: https://doi.org/10.1038/35030000

Fudali, S., Sobczak, M., Janakowski, S., Griesser, M., Grundler F.M.W., Golinowski. W., 2008. Ex- pansins are among plant cell wall modifying agents specifically expressed during development of nematode-induced syncytia. Plant Signaling & Behavior, 3 (11), 969–971. DOI: https://doi.org/10.4161/psb.6169

Gao, B.L., Allen, K.D., Davis, E.L., Baum, T.J., Hussey, R.S., 2004. Developmental expression and biochemical properties of a beta-1,4-endoglucanase family in the soybean cyst nematode, Hete- rodera glycines. Molecular Plant Pathology, 5, 93–104. DOI: https://doi.org/10.1111/j.1364-3703.2004.00209.x

Gerič Stare, B., Lamovšek, J., Širca, S., Urek, G., 2012 Assessment of sequence variability in pu- tative parasitism factor, expansin (expB2) from diverse populations of potato cyst nematode Globodera rostochiensis. Physiological and Molecular Plant Pathology, in press (doi:10.1016/j. pmpp.2012.04.002) DOI: https://doi.org/10.1016/j.pmpp.2012.04.002

Kent, W.J., Zahler, A.M., 2000. Conservation, regulation, synteny, and introns in a large-scale C. briggsae-C. elegans genomic alignment. Genome Research, 10, 1115–1125. DOI: https://doi.org/10.1101/gr.10.8.1115

Kyndt, T., Haegeman, A., Gheysen, G., 2008. Evolution of GHF5 endoglucanase gene structure in plant-parasitic nematodes: no evidence for an early domain shuffling event. BMC Evolutionary Biology, 8, 305. DOI: https://doi.org/10.1186/1471-2148-8-305

Popeijus, H., Blok, V.C., Cardle, L., Bakker, E., Phillips, M.S., Helder, J., Smant, G., Jones, J.T., 2000 Analysis of genes expressed in second stage juveniles of the potato cyst nematodes Globodera rostochiensis and G. pallida using the expressed sequence tag approach. Nematology, 2000, 2 (5), 567–574. DOI: https://doi.org/10.1163/156854100509358

Qin, L., Kudla, U., Roze E.H.A., Goverse, A., Popeijus, H., Nieuwland, J., Overmars, H., Jones, J.T., Schots, A., Smant, G., Bakker, J., Helder, J., 2004. A nematode expansin acting on plants, Nature, 427, 30. DOI: https://doi.org/10.1038/427030a

Širca, S., Gerič Stare, B., Strajnar, P., Urek, G., 2010, PCR-RFLP diagnostic method for identifying Globodera species in Slovenia. Phytopathologia Mediterranea, 49, 361–369.

Širca, S., Gerič Stare, B., Strajnar, P., Urek, G., Miklič Lautar, I., 2012. First report of the pale potato cyst nematode Globodera pallida from Slovenia. Plant disease, 96, 773. DOI: https://doi.org/10.1094/PDIS-01-12-0066-PDN

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Published

01.07.2012

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Original Research Paper

How to Cite

Gerič Stare, B., Širca, S., & Urek, G. (2012). Lack of introns in putative parasitism factor gene, expansin (expB2) from pale potato cyst nematode Globodera pallida. Acta Biologica Slovenica, 55(1), 45-48. https://doi.org/10.14720/abs.55.1.15526