Extraction of DNA from different sample types – a practical approach for GMO testing

Authors

  • Jana Žel
  • Tina Demšar
  • Dejan Štebih
  • Mojca Milavec
  • Kristina Gruden

DOI:

https://doi.org/10.14720/abs.58.2.15611

Keywords:

Extraction methods, Genetically modified organisms (GMO), Decision-making system, GMO testing, NucleoSpin® Food, Cetyltrimethylammonium bromide (CTAB)

Abstract

Current methods based on DNA targets for the detection, identification and quantification of genetically modified organisms (GMOs) involve extraction of the DNA. Different extraction procedures have been developed for the great variety of samples from food, feed, seeds and particular plant parts. This makes the operation of routine analytical laboratories complex and workloads heavy. Here we present a decision-making system, developed over many years of GMO testing on different samples, that result in the application of only a few extraction methods for the majority of samples. Developed decision-making system enables quicker and more cost effective testing of GMOs. In addition, the performance of DNA extraction resulting from the use of the selected extraction methods is presented for use in subsequent testing of GMOs by real time PCR methods. This approach can be used as a model for similar systems based on nucleic acid analysis in food, feed, seeds and plants.

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Published

01.12.2015

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Section

Original Research Paper

How to Cite

Žel, J., Demšar, T., Štebih, D., Milavec, M., & Gruden, K. (2015). Extraction of DNA from different sample types – a practical approach for GMO testing. Acta Biologica Slovenica, 58(2), 61-75. https://doi.org/10.14720/abs.58.2.15611

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