The effect of selenium and iodine on selected biochemical and morphological characteristics in kohlrabi sprouts (Brassica oleracea L. var. gongylodes L.)

Authors

  • Amela Osmić
  • Aleksandra Golob
  • Mateja Germ

DOI:

https://doi.org/10.14720/abs.60.1.15668

Keywords:

kohlrabi, sprouts, selenium, iodine

Abstract

Selenium (Se) and iodine (I) are essential elements for humans and animals, while their essential role for plants has not been established yet. There is also very little information about the interaction between selenium and iodine in plants. The aim of our research was to determine the effect of different forms of Se, I and their combinations on selected biochemical and morphological characteristics of the kohlrabi sprouts (Brassica oleracea L. var. gongylodes L.). Sprouts were grown from seeds, which were soaked in different solutions of selenite, selenate, iodide, iodate and their combinations. We measured the content of chlorophyll a and b, carotenoids, anthocyanins, and UV-A and UV-B absorbing substances. We also measured potential photochemical efficiency of photosystem II (PS II). At the end of the experiment the weight and height of the sprouts were measured. In order to compare the results the entire experiment was carried out twice. Different chemical forms of Se and I, and combinations did not significantly affect the number of sprouts that germinated from seeds. The various chemical forms of Se and I, and combinations differently affected on the amount of pigments in the kohlrabi sprouts. Potential photochemical efficiency of PS II was close to theoretical maximum 0.83.

References

Blasco, B., Ríos, J.J., Cervilla, L.M., Sánchez-Rodríguez, E., Ruiz, J.M., Romero, L., 2008. Iodine biofortification and antioxidant capacity of lettuce: potential benefits for cultivation and human health. Annals of Applied Biology, 152 (3), 289–299. DOI: https://doi.org/10.1111/j.1744-7348.2008.00217.x

Blasco, B., Ríos, J. J., Leyva, R., Melgarejo, R., Constán-Aguilar, C., Sánchez-Rodríguez, E., RubioWilhelmi M.M., Romero L., Ruiz, J. M., 2011. Photosynthesis and metabolism of sugars from lettuce plants (Lactuca sativa L. var. longifolia) subjected to biofortification with iodine. Plant DOI: https://doi.org/10.1007/s10725-011-9583-0

Growth Regulation, 65 (1), 137-143.

Caldwell, M. M., 1968. Solar UV radiation as an ecological factor for alpine plants. Ecological Monographs 38, 243–268. DOI: https://doi.org/10.2307/1942430

Drumm, H., Mohr, H., 1978. Mode of interaction between blue (UV) light photoreceptor and phytochrome in anthocyanin formation of Sorghum seedling. Photochemistry and photobiology 27 (2), 241-248. DOI: https://doi.org/10.1111/j.1751-1097.1978.tb07595.x

Germ, M., Kreft, I., Stibilj, V., Urbanc-Berčič, O., 2007. Combined effects of selenium and drought on photosynthesis and mitochondrial respiration in potato. Plant Physiology and Biochemistry, 45 (2), 162-167. DOI: https://doi.org/10.1016/j.plaphy.2007.01.009

Germ, M., Kacjan Maršić, N., Turk, J., Pirc, M., Golob, A., Jerše, A., … Stibilj, V., 2015. The effect of different compounds of selenium and iodine on selected biochemical and physiological characteristics in common buckwheat and pumpkin sprouts. Acta biologica Slovenica, 58 (1), 35-44.

Hajiboland, R., Keivanfar, N., 2012. Selenium supplementation stimulates vegetative and reproductive growth in canola (Brassica napus L.) plants. Acta agriculturae Slovenica, 99 (1), 13-19. DOI: https://doi.org/10.2478/v10014-012-0002-7

Hasanuzzaman, M., Nahar, K., Fujita, M., 2014. Silicon and selenium: two vital trace elements that confer abiotic stress tolerance to plants. In: Ahmad, P., (ed.): Emerging Technologies and Management of Crop Stress Tolerance, Volume 1. Amsterdam, Elsevier, pp. 377-422. DOI: https://doi.org/10.1016/B978-0-12-800876-8.00016-3

Hawrylak-Nowak, B., 2008. Changes in anthocyanin content as indicator of maize sensitivity to selenium. Journal of Plant Nutrition, 31, 1232-1242. DOI: https://doi.org/10.1080/01904160802134962

Jerše, A., Kacjan-Maršić, N., Šircelj, H., Germ, M., Kroflič., A., Stibilj, V. Seed soaking in I and Se solutions increases concentrations of both elements and changes morphological and some physiological parameters of pea sprouts, Plant Physiology et Biochemistry (2017), doi: 10.1016/j.plaphy.2017.06.009. DOI: https://doi.org/10.1016/j.plaphy.2017.06.009

Khare, M., Guruprasad, K.N., 1993. UV-B-induced anthocyanin synthesis in maize regulated by FMN and inhibitors of FMN photoreactions. Plant Science, 91 (1), 1-5. DOI: https://doi.org/10.1016/0168-9452(93)90182-Y

Krzepilko, A., Zych-Wezyk, I., Swiecilo, A., Molas, J., Skwarylo-Bednarz, B., 2016. Effect of iodine biofortification of lettuce seedlings on their mineral composition and biological quality. Journal of elementology, 21 (4), 1071-1080. DOI: https://doi.org/10.5601/jelem.2015.20.4.1022

Kuznetsov Vas, V., Kholodova, V.P., Kuznetsov Vi., V., Yagodin, B.A., 2003. Selenium regulates the water status of plants exposed to drought. Doklady Biological Sciences, 390 (5), 266-268. DOI: https://doi.org/10.1023/A:1024426104894

Landini, M., Gonzali, S., Perata, P., 2011. Iodine biofortification in tomato. Journal of Plant Nutrition and Soil Science, 174 (3), 480-486. DOI: https://doi.org/10.1002/jpln.201000395

Pirc, S., Šajn, R., 1997. The role of geochemistry in determining the chemical burden of the environment. In: Project “European year of Environmental Protection 1995”. Chemicalisation of life and the environment – how far? Slovene Ecological Society: 165-185

Pongrac, P., Potisek, M., Fraś, A., Likar, M., Budič, B., Myszka, K., … Kreft, I., 2016. Composition of mineral elements and bioactive compounds in tartary buckwheat and wheat sprouts as affected by natural mineral-rich water. Journal of Cereal Science, 69, 9-16. DOI: https://doi.org/10.1016/j.jcs.2016.02.002

Smoleń, S., Kowalska, I. in Sady, W., 2014. Assessment of biofortification with iodine and selenium of lettuce cultivated in the NFT hydroponic system. Scientia Horticulturae, 166, 9-16. DOI: https://doi.org/10.1016/j.scienta.2013.11.011

Schiavon, M., Warzea Lima, L., Jiang, Y., Hawkesford, J.M., 2017. Effects of Selenium on Plant Metabolism and Implications for Crops and Consumers. In: Pilon-Smits, E.A.H, Winkel, L.H.E and Lin, Z.-Q., (eds.): Selenium in plants, pp. 257-275. Springer International Publishing. DOI: https://doi.org/10.1007/978-3-319-56249-0_15

Schreiber, U., Bilger, W., and Neubauer, C., 1995. Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze, E.D. and Caldwell, M.M., (eds.): Ecophysiology of photosynthesis, pp. 49-70. Springer Berlin Heidelberg. DOI: https://doi.org/10.1007/978-3-642-79354-7_3

Strzetelski, P., Smoleń, S., Rożek, S., Sady, W., 2010. The effect of diverse iodine fertilization on nitrate accumulation and content of selected compounds in radish plants (Raphanus sativus L.). Acta Scientiarum Polonorum, Hortorum Cultus, 9 (2), 65-73.

Tadina, N., Germ, M., Kreft, I., Breznik, B., Gaberščik, A., 2007. Effects of water deficit and selenium on common buckwheat (Fagopyrum esculentum Moench.) plants. Photosynthetica, 45 (3), 472-476. DOI: https://doi.org/10.1007/s11099-007-0080-7

White, P.J., Broadley, M.R., 2009. Biofortification of crops with seven mineral elements often lacking in human diets – iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytologist, 182 (1), 49-84. DOI: https://doi.org/10.1111/j.1469-8137.2008.02738.x

Zhu, Y. G., Huang, Y., Hu, Y., Liu, Y., Christie, P., 2004. Interactions between selenium and iodine uptake by spinach (Spinacia oleracea L.) in solution culture. Plant and Soil, 261 (1), 99-105. DOI: https://doi.org/10.1023/B:PLSO.0000035539.58054.e1

Yao, X., Chu, J., Ba, C., 2010. Antioxidant responses of wheat seedlings to exogenous selenium supply under enhanced ultraviolet-B. Biological Trace Elements Research 136 (1), 96-105. DOI: https://doi.org/10.1007/s12011-009-8520-9

Yao, X., Chu, J., Cai, K., Liu, L., Shi, J., Geng, W., 2011. Silicon improves the tolerance of wheat seedlings to ultraviolet-B stress. Trace Elements Research 143 (1), 507-517. DOI: https://doi.org/10.1007/s12011-010-8859-y

Xue, T., Hartikainen, H., Piironen, V., 2001. Antioxidative and growth-promoting effect of selenium on senescing lettuce. Plant and Soil, 237, 55-61. DOI: https://doi.org/10.1023/A:1013369804867

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Published

01.07.2017

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Original Research Paper

How to Cite

Osmić, A., Golob, A., & Germ, M. (2017). The effect of selenium and iodine on selected biochemical and morphological characteristics in kohlrabi sprouts (Brassica oleracea L. var. gongylodes L.). Acta Biologica Slovenica, 60(1), 41-51. https://doi.org/10.14720/abs.60.1.15668

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