Response of macrophyte Berula erecta to low concentrations of NaCl in vitro


  • Špela Mechora
  • Jana Ambrožič Dolinšek



NaCl, Berula erecta, photochemical efficiency, growth parameters, pigments


Macrophyte Berula erecta, grown in tissue culture, was exposed to various low concentrations of NaCl in the water (1–100 mg L-1). Added NaCl had a positive effect on plant’s growth and development. The number of shoots increased, as well as the length of the roots. The lowest concentration (1 mg L-1) increased photochemical efficiency of photosystem II (Fv/Fm) while the highest (100 mg L-1) slightly decreased it. Chlorophyll content was negatively affected by NaCl addition after 3 weeks. Carotenoid and anthocyanin levels were firstly raised and later lowered in NaCl treatment comparing to control. Overall, added NaCl had no negative effect on plants morphology, while decreased amount of pigments was observed.


Al-Karaki, G.N., 2000. Growth, water use efficiency, and sodium and potassium acquisition by tomato cultivars grown under salt stress. Journal of Plant Nutrition, 23, 1–8. DOI:

Amira, M.S., Qados, A., 2011. Effect of salt stress on plant growth and metabolism of bean plant Vicia faba (L.). Journal of the Saudi Society of Agricultural Sciences, 10, 7–15. DOI:

Chen, F., Chen, S., Guo, W., Ji, S., 2003. Salt tolerance identification of three species of chrysanthemums. Acta Horticulturae, 618, 299–305. DOI:

Drumm, H., Mohr, H., 1978. The mode of interaction between blue (UV) lightphotoreceptor and phytochrome in anthocyanin formation of the Sorghumseedling. Photochemistry and Photobiology, 27, 241–248. DOI:

Fargašová, A., 1998. Root growth inhibition, photosynthetic pigments production, andmetal accumulation in Sinapis alba as the parameters for trace metals effect determination. Bulletin of Environmental Contamination and Toxicology, 61, 762–769. DOI:

Hanci, F., Cebeci, E., Uysal, E., Dasgan, H.Y., 2016. Effects of salt stress on some physiological parameters and mineral element contents of onion (Allium cepa L.) plants. Acta Horticulturae, 1143, 179-186. DOI:

Lee, M.K., van Iersel, M.W., 2008. Sodium chloride effects on growth, morphology, and physiology of Chrysanthemum (Chrysanthemum ·morifolium). HortScience, 43,1888–1891. DOI:

Lichtenthaler, H.K., Buschmann, C., 2001. Extraction of photosynthetic tissues:chlorophylls and carotenoids. In: Current Protocols in Food Analytical Chemistry. John Wiley and Sons, Madison, pp F4.2.1-F4.2.6 [Nr. 106]. DOI:

Shabala, S., Munns, R., 2017. Salinity stress: Physiological constraints and adaptive mechanisms. 24-63. In: Shabala, S. (ed.): Plant stress physiology, 2nd edition. CABI International, Boston. Schreiber, U., Bilger, W., Neubauer, C., 1995. Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze, E.D., Caldwell, M.M. (eds.): Ecophysiology of Photosynthesis. Springer Verlag, Berlin, Heidelberg, New York, pp. 49–70. DOI:

Smit, B., Varun, M., Clement, O.O., Manoj, S.P., 2017. Growth and physiological responses of Atriplex lentiformis to variable levels of salinity. International Journal of Botany Studies, 2, 56-62.

Tort, N., Turkyilmaz, B., 2004. A physiological investigation on the mechanisms of salinity tolerance in some barley culture forms. Journal of Forest Science, 27, 1–16.

Winkel-Shirley, B., 2002. Biosynthesis of flavonoids and effects of stress. Current Opinion in Plant Biology, 5, 218–223. DOI:

Willey, N., 2016. Environmental Plant Physiology. Garland Science, New York p. 201–225.






Original Research Paper

How to Cite

Mechora, Špela, & Ambrožič Dolinšek, J. (2017). Response of macrophyte Berula erecta to low concentrations of NaCl in vitro. Acta Biologica Slovenica, 60(2), 41-46.

Similar Articles

1-10 of 77

You may also start an advanced similarity search for this article.