Identification of alien Fallopia taxa using molecular methods

Authors

  • Simona Strgulc Krajšek
  • Mersiha Bjelić
  • Sabina Anžlovar

DOI:

https://doi.org/10.14720/abs.60.2.15686

Keywords:

Fallopia, invasive species, Slovenia, hybridization, plastid DNA, trnK, microsatellite locus, KW6, RFLP

Abstract

The non-native species of knotweeds (Fallopia sect. Reynoutria) are morphologically very similar and it is often difficult to distinguish between the hybrid F. ×bohemica and parental taxa, F. japonica and F. sachalinensis. To distinguish 30 samples of knotweeds, collected in Slovenia, we used PCR RFLP analysis of the trnK intron of plastid DNA in combination with the amplification of microsatellite nuclear locus KW6, which is a specific diagnostic marker for F. sachalinensis. We established that the combination of both markers unambiguously identifies the following samples: F. japonica (var. japonica), F. sachalinensis and F. ×bohemica. Based
on described molecular markers we confirmed that the maternal parent of the taxon F. ×bohemica was F. japonica for all analysed hybrids. In addition, two species from Fallopia sect. Sarmentosae (F. baldschuanica and F. multiflora) were also analysed.
Both could be distinguished from species of Fallopia sect. Reynoutria, but for the discrimination between them, some other markers should be used.

References

Bailey, J.P., 2001. Fallopia × conollyana the railway-yard knotweed. Watsonia, 23, 539–541.

Bailey, J.P., 2003. Japanese knotweed s. l. at home and abroad. In: Child, L., Brock, J.H., Prach, K., Pyšek, P., Wade, P.M., Williamson, M., (eds.): Plant invasions – ecological threats and management solutions. Leiden, Backhuys Publishers, pp. 183–196.

Bailey, J.P., Bímová, K., Mandák, B., 2009. Asexual spread versus sexual reproduction and evolution in Japanese knotweed s. l. sets the stage for the “Battle of the clones”. Biological invasions, 11, 1189–1203. DOI: https://doi.org/10.1007/s10530-008-9381-4

Bailey, J.P., Bímová, K., Mandák, B., 2007. The potential role of polyploidy and hybridisation in the further evolution of the highly invasive Fallopia taxa in Europe. Ecological Research, 22, 920–928. DOI: https://doi.org/10.1007/s11284-007-0419-3

Bailey, J.P., Conolly A.P., 2000. Prize-winners to pariahs – a history of Japanese knotweed s. l. (Polygonaceae) in the British Isles. Watsonia, 23, 93–110.

Balant, M., Strgulc Krajšek, S, Jogan, N., 2015. Potentially invasive species Fallopia baldschuanica and F. multiflora (Polygonaceae) in Slovenia. In: Bogdanović S., Jogan N. (eds.): Book of abstracts – 6th Balkan Botanical Congress, Rijeka, Sept. 14–18, 2015. p. 48.

Bímová, K., Mandák, B., Pyšek, P., 2003. Experimental study of vegetative regeneration in four invasive Reynoutria taxa (Polygonaceae). Plant Ecology, 166, 1–11.

Demesure, B., Sodzi, N., Petit, R.J., 1995. A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Molecular Ecology, 4, 129–131. DOI: https://doi.org/10.1111/j.1365-294X.1995.tb00201.x

Forman, J.R., Kesseli, V., 2003. Sexual reproduction in the invasive species Fallopia japonica (Polygonaceae). American Journal of Botany, 90, 586–592. DOI: https://doi.org/10.3732/ajb.90.4.586

Grimsby, J.L., Kesseli, R., 2010. Genetic composition of invasive Japanese knotweed s. l. in the United States. Biological Invasions, 12, 1943–1946. DOI: https://doi.org/10.1007/s10530-009-9602-5

Grimsby, J.L., Tsirelson, D., Gammon, M.A., Kesseli, R., 2007. Genetic diversity and clonal vs. sexual reproduction in Fallopia spp. (Polygonaceae). American Journal of Botany, 94(6), 957–964. DOI: https://doi.org/10.3732/ajb.94.6.957

Hollingsworth, M.L., Bailey, J.P., 2000. Evidence for massive clonal growth in the invasive Fallopia japonica (Japanese knotweed). Botanical Journal of the Linnean Society., 133, 463–472. DOI: https://doi.org/10.1006/bojl.2000.0359

Mandák, B., Pyšek, P., Lysák, M., Suda, J., Krahulcová, K., Bímová, K., 2003. Variation in DNA-ploidy levels of Reynoutria taxa in the Czech Republic. Annals of Botany, 92, 265–272. DOI: https://doi.org/10.1093/aob/mcg141

Strgulc Krajšek, S., Dolenc Koce, J., 2015. Sexual reproduction of knotweeds (Fallopia sect. Reynoutria) in Slovenia. Preslia, 87, 17–30.

Strgulc Krajšek, S., Jogan, N., 2011. The genus Fallopia Adans. in Slovenia (Rod Fallopia Adans. v Sloveniji). Hladnikia, 28, 17–40.

Suda, J., Trávníček, P., Mandák, B., Berchová-Bímová, K., 2010. Genome size as a marker for identifying the invasive alien taxa in Fallopia section Reynoutria. Preslia, 82, 97–106.

Tiébré, M.S., Vanderhoeven, S., Saad, L., Mahy, G., 2007. Hybridization and sexual reproduction in the invasive alien Fallopia (Polygonaceae) complex in Belgium. Annals of Botany, 99, 193–203. DOI: https://doi.org/10.1093/aob/mcl242

Wilson, E.O., 2001. The diversity of life, New edition. Penguin Press Science, London. 432 pp.

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Published

01.12.2017

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Original Research Paper

How to Cite

Strgulc Krajšek, S., Bjelić, M., & Anžlovar, S. (2017). Identification of alien Fallopia taxa using molecular methods. Acta Biologica Slovenica, 60(2), 47-54. https://doi.org/10.14720/abs.60.2.15686

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