Immune system in crustaceans: a presentation of research with terrestrial crustacean Porcellio scaber

Authors

  • Andraž Dolar
  • Damjana Drobne
  • Rok Kostanjšek
  • Anita Jemec Kokalj

DOI:

https://doi.org/10.14720/abs.64.1.15759

Keywords:

encapsulation, haemocyte, melanization, nodulation, phagocytosis, signal pathway

Abstract

The immune system is crucial to recognise and distinguish between self and nonself, and react to challenges posed by the environment. We present a review on the innate immunity of crustaceans, in particular terrestrial crustacean woodlice Porcellio scaber. The main effectors of immunity in crustaceans are haemocytes which carry out the cellular immune processes and synthesise humoral defence components. We described three types of haemocytes in P. scaber: semigranulocytes (65% of freely
circulating haemocytes), granulocytes (17%) and hyalinocytes (18%). The average density of free circulating haemocytes is 3.50 ± 0.19 x 106 cells/mL. Semigranulocytes and granulocytes are both oval shaped with a similar diameter (11.2 ± 0.4 µm and 12.0 ± 0.3 µm, respectively), but granulocytes have a higher density of granules which are also larger than in the case of semigranulocytes. Hyalinocytes are round, agranular and smaller (8.1 ± 0.3 µm). Two types of hyalinocytes were discovered. One subtype is of particular interest, as it has a very large nucleus with dominating euchromatine resembling properties of the stem progenitor cells. We have implemented new methods to measure humoral components in the haemolymph of P. scaber. These are: phenoloxidase like activity, nitric oxide levels, antioxidant enzyme activity and alpha2-macroglobulin. The formation of nodules was noted in the case of P. scaber infection with bacteria Rhabdochlamydia porcellionis. Our future research will be focused to investigate the immune response of P. scaber at the proteome and transcriptome level.

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01.07.2021

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Original Research Paper

How to Cite

Dolar, A., Drobne, D., Kostanjšek, R., & Jemec Kokalj, A. (2021). Immune system in crustaceans: a presentation of research with terrestrial crustacean Porcellio scaber. Acta Biologica Slovenica, 64(1), 18-35. https://doi.org/10.14720/abs.64.1.15759

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