Stinkwort (Dittrichia graveolens) organic extracts as potential biofungicides for Fusarium poae
DOI:
https://doi.org/10.14720/abs.63.2.15934Keywords:
Dittrichia graveolens, Fusarium poae, antifungal activity, seed germinationAbstract
In the present study, we tested the antifungal activity of ethanol, methanol and acetone extracts of stinkwort against pathogenic fungus grown from ecological wheat grain, molecularly identified as Fusarium poae. Its susceptibility to the stinkworth extracts was tested in vitro with agardilution method. The results of antifungal effect of the organic stinkwort extracts showed that the growth of F. poae was significantly reduced by these extracts compared to the control and that the antifungal activity is dose-dependent. The methanol extract showed stronger inhibition than the ethanol and acetone ones at all three concentrations. All organic extracts showed a similar antifungal activity against F. poae as a broad-spectrum fungicide azoxystrobin. We tested the effects of stinkwoth extracts on the germination of radish seeds. The methanol extract delayed the germination of the radish seeds during the first 24 hours, but thereafter all three organic extracts had a comparable germination rate as the control seeds. Despite the delayed germination of the radish seeds, the methanol extract did not reduce the final germination rate and at the same time reduced the fungal infection by almost 50%. These results indicate that the methanol stinkwort extract has the potential to be used as a biofungicide in organic farming.
References
Abu Irmaileh, B.E., Salem, N.M., Al Aboudi, A.M.F., Abu Zarqa, M.H., Abdeen, A.O., 2017. Antifungal activity of the stinkwort (Inula graveolens) extracts. Journal of Plant Pathology & Microbiology, 8, 8. DOI: https://doi.org/10.4172/2157-7471.1000417
Aghel, N., Mahmoudabadi, A. Y., Darvishi, L., 2011. Volatile constituents and anti-candida activity of the aerial parts essential oil of Dittrichia graveolens (L.) Greuter grown in Iran. African Journal of Pharmacy and Pharmacology, 5(6), 772-775. DOI: https://doi.org/10.5897/AJPP10.145
Ajao, A.A., Moteetee, A.N., 2015. Tithonia diversifolia (Hemsl) A. Gray. (Asteraceae: Heliantheae), an invasive plant of significant ethnopharmacological importance: A review. South African Journal of Botany, 113, 396-403. DOI: https://doi.org/10.1016/j.sajb.2017.09.017
Alwahshi, K.J., Saeed, E.E., Sham, A., Alblooshi, A.A., Alblooshi, M.M., El-Tarabily, K.A., AbuQamar, S.F., 2019. Molecular identification and disease management of date palm sudden decline syndrome in the United Arab Emirates. International Journal of Molecular Sciences, 20, 923. DOI: https://doi.org/10.3390/ijms20040923
Anžlovar, S., Dolenc Koce, J., 2014. Antibacterial and antifungal activity of aqueous and organic extracts from indigenous and invasive species of Goldenrod (Solidago spp.) grown in Slovenia. Phyton, 54, 135-147.
Anžlovar, S., Likar, M., Dolenc Koce, J., 2017. Antifungal potential of thyme essential oil as a preser- vative for storage of wheat seeds. Acta Botanica Croatica, 76, 64–71. DOI: https://doi.org/10.1515/botcro-2016-0044
Bajpai, V.K., Baek, K.H., Kim, E.S., Han, J.E., Kwak, M., Oh, K., Kim, J.C., Kim, S., Choi, G.J., 2012. In vivo antifungal activities of the methanol extracts of invasive plant species against plant pathogenic fungi. The Plant Pathology Journal, 28 (3), 317-321. DOI: https://doi.org/10.5423/PPJ.NT.04.2012.0056
Bezuneh, T.T., 2015. Phytochemistry and antimicrobial activity of Parthenium hysterophorus L.: A Review. Science Journal of Analytical Chemistry, 3 (3), 30-38. DOI: https://doi.org/10.11648/j.sjac.20150303.11
Cappuccino, N., Arnason, J.T., 2006. Novel chemistry of invasive exotic plants. Biology Letters, 2, 189–193. DOI: https://doi.org/10.1098/rsbl.2005.0433
Chen, P.J., Moore, T., Nesnow, S., 2008. Cytotoxic effects of propiconazole and its metabolites in mouse and human hepatoma cells and primary mouse hepatocytes. Toxicology in Vitro, 22, 1476–1483. DOI: https://doi.org/10.1016/j.tiv.2008.05.001
Das, R.K., Devkota, A., 2018. Antifungal activities and phytochemical screening of two invasive alien species of Nepal. Studies in Fungi, 3(1), 293–301. DOI: https://doi.org/10.5943/sif/3/1/29
Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S., Ju, YH., 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22 (3), 296-302. DOI: https://doi.org/10.1016/j.jfda.2013.11.001
Eloff, J.N., 1998. Which extractant should be used for the screening and isolation of antimicrobial components from plants? Journal of Ethnopharmacology, 60, 1–8. DOI: https://doi.org/10.1016/S0378-8741(97)00123-2
Elshafie, H. S., Gruľová, D., Baranová, B., Caputo, L., De Martino, L., Sedlák, V., Camele, I., De Feo, V., 2019. Antimicrobial activity and chemical composition of essential oil extracted from Solidago canadensis L. growing wild in Slovakia. Molecules, 24, 1206. DOI: https://doi.org/10.3390/molecules24071206
Frajman, B., Kaligarič, M., 2009. Dittrichia graveolens, a new alien species of the Slovenian flora (Dittrichia graveolens, nova tujerodna vrsta slovenske flore). Hladnikia, 24, 35-43.
Grašič, M., Anžlovar, S., Strgulc Krajšek, S., 2016. The impact of aqueous extracts of stinkwort (Dit- trichia graveolens) and false yellowhead (D. viscosa) on germination of selected plant species. Phyton, 56 (2), 293-301.
Gioahin, E., 2016. Antimicrobial activity of Japanese knotweed (Fallopia japonica) essential oils and extracts. MSc Thesis, University of Ljubljana, Biotechnical faculty, Department of food science and technology, 63 pp.
Hladnik, S., 2017. Antifungal activity of aqueous and organic extracts from Goldenrod. MSc Thesis, University of Ljubljana, Faculty of education, Department of biology, 46 pp.
Jankovec, M., 2016. Biological activity of goldenrod (Solidago spp.) essential oil. MSc Thesis, Uni- versity of Ljubljana, Biotechnical faculty, Department of biology, 60 pp.
Karlsson, I., Friberg, H., Kolseth, A.K., Steinberg, C., Persson, P., 2017. Organic farming increases richness of fungal taxa in the wheat phyllosphere. Molecular Ecology, 26: 3424-3436. DOI: https://doi.org/10.1111/mec.14132
Knudesen, M.B., Hockenhull, J., Jensen, D.F., 1995. Biocontrol of seedling diseases of barley and wheat caused by Fusarium culmorum and Bipolaris sorokiniana: Effects of selected fungal antagonists on growth and yield components. Plant Pathology, 44, 467–77. DOI: https://doi.org/10.1111/j.1365-3059.1995.tb01669.x
Lampiri, E., Agrafioti, P., Levizou, E., Athanassiou, C.G., 2020. Insecticidal effect of Dittrichia viscosa lyophilized epicuticular material against four major stored-product beetle species on wheat. Crop Protection, 132, 105095. DOI: https://doi.org/10.1016/j.cropro.2020.105095
Lira-De León, K.I., Ramírez-Mares, M.V., Sánchez-López, V., Ramírez-Lepe, M., Salas-Coronado, R., Santos-Sánchez, N.F. et al., 2014. Effect of crude plant extracts from some Oaxacan flora on two deleterious fungal phytopathogens and extract compatibility with a biofertilizer strain. Frontiers in Microbiology, 5, 383. DOI: https://doi.org/10.3389/fmicb.2014.00383
Liu, G.Y., Nizet, V., 2010. Color me bad: microbial pigments as virulence factors. Trends in Micro- biology, 17, 406–413. DOI: https://doi.org/10.1016/j.tim.2009.06.006
Liu, S., Shao, X., Wei, Y., Li, Y., Xu, F., and Wang, H., 2016. Solidago canadensis L. essential oil vapor effectively inhibits Botrytis cinerea growth and preserves postharvest quality of strawberry as a food model system. Frontiers in Mycrobiology, 7, 1179. DOI: https://doi.org/10.3389/fmicb.2016.01179
Mazandarani, M., Ghafourian, M., Khormali, A., 2014. Ethnopharmacology, antibacterial and antioxidant activity of Dittrichia graveolens (L.) W. Greuter. which has been used as remedies antirheumatic, anti-inflammation and antiinfection against leishmaniasis in the traditional medicine of Gorgan, Iran. Crescent Journal of Medical and Biological Sciences, 1, 125-129.
Mdee, L.K., Masoko, P., Eloff, J.N., 2009. The activity of extracts of seven common invasive plant species on fungal phytopathogens. South African Journal of Botany, 75, 375–379. DOI: https://doi.org/10.1016/j.sajb.2009.02.003
Nogueira, M. S., Decundo, J., Martinez, M., Dieguez, S. N., Moreyra, F., Moreno, M.V., Stenglein, S.A., 2018. Natural contamination with mycotoxins produced by Fusarium graminearum and Fusarium poae in malting barley in Argentina. Toxins, 10, 78. DOI: https://doi.org/10.3390/toxins10020078
Omezzine, F., Ladhari, A., Rinez, A., Haouala, R., 2011. Allelopathic potential of Inula graveolens on crops and weeds. Allelopathy Journal, 28, 63-76.
Pekarskas, J., Sinkevičienė, J., 2015. Effect of biopreparations on seed germination and fungal conta- mination of winter wheat. Biologija, 61 (1), 25-33. DOI: https://doi.org/10.6001/biologija.v61i1.3108
Ponzilacqua, B., Corassin, C. H., Fernandes Oliveira, C. A., 2018. Antifungal activity and detoxification of aflatoxins by plant extracts: Potential for food applications. The Open Food Science Journal, 10, 24–32. DOI: https://doi.org/10.2174/1874256401810010024
Rashmi, S., Rajkumar, H.G., 2011. Preliminary phytochemical analysis and in vitro evaluation of antifungal activity of five invasive plant species against Macrophomina Phaseolina (Tassi) Goid. International Journal of Plant Research, 1 (1), 11-15. DOI: https://doi.org/10.5923/j.plant.20110101.02
Richardson, D.M., Pyšek, P., Rejmanek, M., Barbour, M.G., Panetta, F.D., West, C.J., 2000. Naturali- zation and invasion of alien plants: concepts and definition. Diversity and Distributions, 6, 93–107. Schöneberg, T., Kibler, K., Sulyok, M., Musa, T., Bucheli, T.D., Mascher, F., Bertossa, M., Voegele, R.T., Vogelgsang, S., 2018. Can plant phenolic compounds reduce Fusarium growth and mycotoxin production in cereals? Food Additive and Contaminants, part A 35 (12), 2455–2470. DOI: https://doi.org/10.1080/19440049.2018.1538570
Sellem, I., Chakchouk-Mtibaaa, A., Zaghdenb, H., Smaouia, S., Ennouria, K., Mellouli, L., 2020. Harvesting season dependent variation in chemical composition and biological activities of the essential oil obtained from Inula graveolens (L.) grown in Chebba (Tunisia) salt marsh. Arabian Journal of Chemistry, 13 (3), 4835-4845. DOI: https://doi.org/10.1016/j.arabjc.2020.01.013
Sofou, K., Isaakidis, D., Spyros, A., Büttner, A., Giannis, A. and Katerinopoulos, H.E., 2017. Use of costic acid, a natural extract from Dittrichia viscosa, for the control of Varroa destructor, a parasite of the European honey bee. Beilstein Journal of Organic Chemistry, 13, 952-959. DOI: https://doi.org/10.3762/bjoc.13.96
Stenglein, S. A., 2009. Fusarium poae: a pathogen that needs more attention. Journal of Plant Patho- logy, 91 (1), 25-36.
Shuping, D.S.S., Elof, J.N., 2017. The use of plants to protect plants and food against fungal patho- gens. African Journal of Traditional, Complementary and Alternative Medicines, 14 (4), 120-127. DOI: https://doi.org/10.21010/ajtcam.v14i4.14
Yogev, A., Laor, Y., Katan, J., Hadar, Y., Cohen, R., Medina, S., Raviv, M., 2011. Does organic farming increase soil suppression against Fusarium wilt of melon? Organic agriculture, 1, 203-216. DOI: https://doi.org/10.1007/s13165-011-0016-1
Xia, Y., Sahib, M.R., Amna, A., Opiyo, S.O., Zhao, Z., Gao, Y.G., 2019. Culturable endophytic fungal communities associated with plants in organic and conventional farming systems and their effects on plant growth. Nature Scientific Reports, 9, 1669. DOI: https://doi.org/10.1038/s41598-018-38230-x
Zaidel, D.N.A., Muhamad, I.I., Daud, N.S.M., Muttalib, M.A.A., Khairudd, N., Lazim, N.A.M., 2019. Production of biodiesel from rice bran oil. In: Verma, D., Fortunati, E., Jain, S., Zhang, X.: Biomass, biopolymer-based materials, and bioenergy: construction, biomedical, and other indus- trial applications. Woodhead Publishing Series in Composites Science and Engineering, 409-447. DOI: https://doi.org/10.1016/B978-0-08-102426-3.00018-7
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
