Inter- and intracellular distribution of modulated glutathione in plant tissues

Authors

  • Maria Muller Institute of Plant Physiology, University of Graz, Graz, Austria
  • Bernd Zechmann Institute of Plant Physiology, University of Graz, Graz, Austria
  • Andreja Urbanek Institute of Plant Physiology, University of Graz, Graz, Austria
  • Gunther Zellnig Institute of Plant Physiology, University of Graz, Graz, Austria

DOI:

https://doi.org/10.14720/abs.46.2.16660

Keywords:

glutathione, immulabelling, monochlorobimane, plant tissues, ultrastructure

Abstract

In the present study we want to summarize data dealing with the inter­ and intracellular distribution of glutathione in different plant tissues under various environmental conditions. Monochlorobimane fluorescence of reduced glutathione is described for light microscopical investigations and allows distinguishing between glutathione in cytoplasm and in the nucleus. Further, a specific antibody, which recognizes both the reduced and oxidized form of glutathione, was used in order to demonstrate glutathione in different cell compartments. The labelling showed different glutathione concentrations in the cell compartments with a high staining intensity in mitochondria.

References

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Published

01.12.2003

Issue

Vol. 46 No. 2 (2003): Acta Biologica Slovenica

Section

Review

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

How to Cite

Muller, M., Zechmann, B., Urbanek, A., & Zellnig, G. (2003). Inter- and intracellular distribution of modulated glutathione in plant tissues. Acta Biologica Slovenica, 46(2), 17-20. https://doi.org/10.14720/abs.46.2.16660