Inter- and intracellular distribution of modulated glutathione in plant tissues


  • Maria Muller Institute of Plant Physiology, University of Graz, Graz, Austria
  • Bernd Zechmann Institute of Plant Physiology, University of Graz, Graz, Austria
  • Andreja Urbanek Institute of Plant Physiology, University of Graz, Graz, Austria
  • Gunther Zellnig Institute of Plant Physiology, University of Graz, Graz, Austria



glutathione, immulabelling, monochlorobimane, plant tissues, ultrastructure


In the present study we want to summarize data dealing with the inter­ and intracellular distribution of glutathione in different plant tissues under various environmental conditions. Monochlorobimane fluorescence of reduced glutathione is described for light microscopical investigations and allows distinguishing between glutathione in cytoplasm and in the nucleus. Further, a specific antibody, which recognizes both the reduced and oxidized form of glutathione, was used in order to demonstrate glutathione in different cell compartments. The labelling showed different glutathione concentrations in the cell compartments with a high staining intensity in mitochondria.


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How to Cite

Muller, M., Zechmann, B., Urbanek, A., & Zellnig, G. (2003). Inter- and intracellular distribution of modulated glutathione in plant tissues. Acta Biologica Slovenica, 46(2), 17-20.

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