Organization of interphase microtubules and actin filaments in spruce callus cells after glutathione treatment


  • Andreja Urbanek Institute of Plant Physiology, Karl-Franzens-University Graz, Graz, Austria
  • Bernd Zechmann Institute of Plant Physiology, Karl-Franzens-University Graz, Graz, Austria
  • Günther Zellnig Institute of Plant Physiology, Karl-Franzens-University Graz, Graz, Austria
  • Maria Muller Institute of Plant Physiology, Karl-Franzens-University Graz, Graz, Austria



glutathione, spruce callus cells, microtubules, actin filaments


Changes in the distribution of microtubules (MT) and actin filaments were examined in suspension-cultured spruce cells [Picea abies (L.) KARST.] that were exposed to different concentrations (500 and I 000 µM) of exogenously applied reduced glutathione (GSH). Using fluorescence microscopy the MT were visualized with monoclonal anti-tubulin antibodies and actin filaments were stained with rhodamin labelled phalloidin (RLP). GSH-treated callus cells showed modifications on the form and arrangement of both cytoskeletal elements, when compared to the control.


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Original Research Paper

How to Cite

Urbanek, A., Zechmann, B., Zellnig, G., & Muller, M. (2003). Organization of interphase microtubules and actin filaments in spruce callus cells after glutathione treatment. Acta Biologica Slovenica, 46(2), 29-35.

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