Organization of interphase microtubules and actin filaments in spruce callus cells after glutathione treatment

Andreja Urbanek; Bernd Zechmann; Günther Zellnig; Maria Muller

doi:10.14720/abs.46.2.16666

Authors

Andreja Urbanek Institute of Plant Physiology, Karl-Franzens-University Graz, Graz, Austria
Bernd Zechmann Institute of Plant Physiology, Karl-Franzens-University Graz, Graz, Austria
Günther Zellnig Institute of Plant Physiology, Karl-Franzens-University Graz, Graz, Austria
Maria Muller Institute of Plant Physiology, Karl-Franzens-University Graz, Graz, Austria

DOI:

https://doi.org/10.14720/abs.46.2.16666

Keywords:

glutathione, spruce callus cells, microtubules, actin filaments

Abstract

Changes in the distribution of microtubules (MT) and actin filaments were examined in suspension-cultured spruce cells [Picea abies (L.) KARST.] that were exposed to different concentrations (500 and I 000 µM) of exogenously applied reduced glutathione (GSH). Using fluorescence microscopy the MT were visualized with monoclonal anti-tubulin antibodies and actin filaments were stained with rhodamin labelled phalloidin (RLP). GSH-treated callus cells showed modifications on the form and arrangement of both cytoskeletal elements, when compared to the control.

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