Respiration rate and respiratory electron transport system (ETS) activity of chironomid larvae from mountain lakes (NW Slovenia)

Authors

  • Tatjana Simic National Institute of Biology, Ljubljana, Slovenia

DOI:

https://doi.org/10.14720/abs.47.2.16982

Keywords:

Chironomidae, Diptera, benthos, ETS/R ratio, ETS activity, respiration, metabolism

Abstract

Respiration rate (R) and electron transport system (ETS) activity were measured in larvae of four chironomid species, Chironomus thummi (Kieffer), Paratanytarsus austriacus (Kieffer), Procladius (Holotanypus) spp., and Zavrelimyia barbatipes (Kieffer) from three mountain lakes. ETS activity and res­ piration rate, measured at a standard temperature of 20 °C, differed significantly between species (ANOVA: p<0.001). ETS activity ranged from 10.3 µL 02 mg-1 DW h-1 for Zavrelimyia to 27.6 µL 02 mg1DW h-1 for Paratanytarsus. The lowest respiration rate was observed for Procladius (4.0 µL 02 mg·1DW h-1) and the high­ est for Paratanytarsus (12.4 µL 02 mg·1DW h-1). ETS activity and respiration rate of chironomids decreased with increasing size of the animals (p<0.001); b values for ETS activity and respiration rate were 0.82 and 0.66, respectively. Respiration rate correlated significantly with ETS activity for all four species investigated. This study revealed that the ETS/R ratio differed between species (ANOVA: p<0.001). A low ratio (0.98) was determined for Zavrelimyia, while Procladius showed the highest value (3.88). The differences can be explained by body size, and the eco­ logical preference and tolerance of each chironomid species.

References

BAIRLEIN F. 1989: The respiration of Chironomus-larvae (Diptera) from deep and shallow waters under environmental hypoxia and at different temperatures. Arch. Hydrobiol. 115 (4): 523-536.

BAMSTEDT U. 1980: ETS activity as an estimator of respiratory rate of zooplankton populations. The sig­ nificance of variations in environmental factors. J. Exp. Mar. Biol. Ecol. 42: 267-283.

BORGMANN U. 1978: The effect of temperature and body size on electron transport system activity in freshwater zooplankton. Can. J. Zoo!. 56: 634-642.

CAMMEN L. M., S. CORWIN & J.P. CHRISTENSEN 1990: Electron transport system (ETS) activity as a meas­ ure ofbenthic macrofaunal metabolism. Mar. Ecol. Prog. Ser. 65: 171-182.

DEL GIORGIO P. A. 1992: The relationship between ETS (electron transport system) activity and oxygen consumption in lake plankton: a cross-system calibration. J. Plankton Res. 14: 1723-1741.

ERMAN D. C. & W. T. HELM 1970: Estimating oxygen consumption from body length for some Chironomid larvae. Hydrobiologia 36 (3-4): 505-512.

FANSLOW D. L., T. F. NALEPA & T. H. JOHENGEN 2001: Seasonal changes in the respiratory electron trans­ po1t system (ETS) and respiration rate of the zebra mussel, Dreissena polymorpha in Saginaw Bay, Lake Huron. - Hydrobiologia 448: 61-70.

FlTIKAU E. J. & S.S. ROBACK 1983: The larvae ofTanypodinae (Diptera: Chironomidae) of the Holarctic region- Keys and diagnosis. In Wiederholm T. (Ed.) Chironomidae of the Holarctic region. Keys and diagnosis. Part 1 - Larvae. Ent. Scand. Suppl. 19: 33-110.

GNAIGER, E. 1983: The twin-flow microrespirometer and simultaneous calorimetry. In: GNAIGER E. & H. FORSTNER (Eds.): Polarographic Oxygen Sensors, Aquatic and Physiological Applications. Springer-Verlag Berlin Heidelberg New York, pp. 134-166.

G.-Tóth. 1999: Activitat des electronentransportsystems. In: w. VON TDMPL!Ng & G. FRIEDRICH (Eds.): Biologishe Gewasseruntersuchung. Methoden der Biologische Wasseruntersuchung 2: Gustav Fisher Ver!., Jena, Stuttgart, Lubeck, Ulm, pp. 465-473.

G.-Tóth L., z. LANGE, J. PADISAK & E. VARGA 1994: Terminal electron transport system (ETS)-activity in the sediment of Lake Balaton, Hungary. Hydrobiologia 281 (3): 129-139.

G.-Tóth L., M. SZAB6 & D. WEBB 1995: Adaptation of the tetrazolium reduction test for the measurement of the electron transport system (ETS) activity during embryonic development of medaka. J. Fish Biol. 46: 835-844.

HAMBURGER K. & P. C. DALL 1990: The respiration of common benthic invertebrate species from the shal­low littoral zone of Lake Esrom, Denmark. Hydrobiologia 199: 177-130.

HAMBURGER K., P. C. DALL & C. LINDEGAARD 1994: Energy metabolism of Chironomus anthracinus (Diptera: Chironomidae) from the profundal zone of Lake Esrom, Denmark, as a function of body size, temperature and oxygen concentration. Hydrobiologia 294: 43-50.

JAMES M. R. 1987: Respiratory rates in cladoceran Ceriodaphnia dubia in lake Rotiongaio, a monomictic lake. J. Plankton Res. 9: 573-578.

JONASS0N P. M., C. LINDEGAARD & K. HAMBURGER 1990: Energy budget of Lake Esrom. Verh. int. Ver. Limnol. 24: 632-640.

KENNER R. A. & S. I. AHMED 1975: Measurements of electron transport activities in marine phytoplank­ ton. Mar. Biol. 33: 119-127.

KING F. D. & T. T. PACKARD 1975: Respiration and respiratory electron transport system in marine zoo­ plankton. Limnol. Oceanogr. 20: 846-854.

K0NSTANTINOV A. S. 1971: Ecological factors affecting respiration in chironomid larvae. Limnologica (Berlin) 8 (1):127-134.

LAMPERT w. 1984: The measurement of respiration. In: DOWNING J. A. & F. H. RIGLER (Eds.), A manual on methods for the assessment of secondary productivity in fresh water. IPB Handbook 17, sec­ ond edition, Blackwell Scientific Publications, 413-468.

LINDEGAARD C. 1994: The role of zoobenthos in energy flow in two shallow lakes. Hydrobiologia 275/276: 313-322.

MERRIT R.W. & K. W. CUMMINS 1984: Introduction to the aquatic insects of North America. Kendall/Hunt, Dubuque, Iowa, pp. 550-652.

MusK6 I. B., L. G.-T6TH & E. SzAB6 1995: Respiration and respiratory electron transport system (ETS) activity of two amphipods: Corophium curvispinum G. 0. Sars and Gammarus fossarum Koch. Pol. Arch. Hydrobiol. 42: 547-558.

MUSKE I. B., L. G.-Tóth & E. SzAB6 1998: Respiratory energy loss of Corophium curvispinum (Crustacea: Amphipoda) in Lake Balaton (Hungary) during the vegetation period. Verh. Internat. Verein. Limnol. 26: 2107-2114.

OWENS T. G. & F. D. KING 1975: The measurement of respiratory electron transport system activity in marine zooplankton. Mar. Biol. 30: 27-36.

PACKARD T. T. 1971: The measurement of respiratory electron-transport activity in marine phytoplankton. J. Mar. Res. 29 (3): 235-244.

PACKARD T. T. 1985: Measurement of electron transport activity of microplankton. In: JANNAS H. & P. J. WILLIAMS (Eds): Advances in aquatic microbiology. Academic Press, London 3: 207-261.

PETERS R.H. 1983: The ecological implications of body size. Cambridge University Press, 329 pp. PINDER L. C. V. & F. REISS 1983: The larvae of Chironomidae (Diptera: Chironomidae) of the Holarctic region - Keys and diagnosis. In: WIEDERHOLM T. (Ed.): Chironomidae of the Holarctic region. Keys and diagnosis. Part 1 - Larvae. Ent. Scand. Suppl. 19: 293-435.

RELEXANS J. C. 1996: Measurement of the respiratory electron transport system (ETS) activity in marine sediments: state-of-the-art and interpretation. I. Methodology and review of literature data. Mar. Ecol. Prog. Ser. 136: 277-287.

SIMČIČ T. & A. BRANCELJ 1997: Electron transport system (ETS) activity in five Daphnia species at differ­ ent temperatures. Hydrobiologia 360: 117-125.

SIMČIČ T. & A. BRANCELJ 2000: Energy exploitation in Chirocephalus croaticus (Steuer, 1899) (Crustacea:Anostraca): survival strategy in an intermittent lake. Hydrobiologia 437 (1/3): 157- 163.

SIMČIČ T. & A. BRANCELJ 2001: Seasonal dynamics of metabolic activity of the Daphnia community in Lake Bled (Slovenia). Hydrobiologia 442 (1/3): 319-328.

TREVORS J. T. 1984: The measurement of electron transport system (ETS) activity in freshwater sediment. Water Res. 18 (5): 581-584.

VOSJAN J. H. & K. M. OLANCZUK-NEYMAN 1977: Vertical distribution of mineralization processes in a tidal sediment. Neth. J. Sea Res. 11 (I): 14-23.

VOSJAN J. H. & K. M. OLANCZUK-NEYMAN 1991: Influence of temperature on respiratory ETS-activity of micro-organisms from Admiralty Bay, King George Island, Antarctica. Neth. J. Sea Res. 28 (3): 221-225.

Downloads

Published

01.12.2004

Issue

Section

Original Research Paper

How to Cite

Simic, T. (2004). Respiration rate and respiratory electron transport system (ETS) activity of chironomid larvae from mountain lakes (NW Slovenia). Acta Biologica Slovenica, 47(2), 83-94. https://doi.org/10.14720/abs.47.2.16982

Similar Articles

1-10 of 69

You may also start an advanced similarity search for this article.