Evaluation of the in vitro toxicity and anti-inflammatory activity of the methanolic extract of the leaves of Pistacia lentiscus L. harvested from northwestern Algeria.
DOI:
https://doi.org/10.14720/abs.67.3.19738Keywords:
Anti-inflammatory activity, anti-hemolytic activity, Pistacia lentiscus, methanolic extract, ToxicityAbstract
Medicinal and aromatic plants have been used for thousands of years for their therapeutic properties, to treat various ailments and maintain health. This study was carried out to test the in vitro toxicity and anti-inflammatory activity of the methanolic extract of the leaves of Pistacia lentiscus L., native to the north-western region of Algeria. Toxicity was studied in vitro by the hemolysis test and anti-hemolytic activity on human red blood cells, while anti-inflammatory activity was assessed in vitro by the protein denaturation method. The study of toxicity by hemolysis of red blood cells showed a high rate of hemolysis at a dose of 200 mg/mL, with a hemolytic percentage of around 88.64%, while a high rate of anti-hemolysis was observed at a dose of 25 mg/mL, with an inhibition percentage of around 83.17%. Evaluation of anti-inflammatory activity revealed a high percentage of inhibition of ovalbumin denaturation, reaching 63.53% at the 2000 ug/mL dose, while the lowest percentage was obtained at the 3500 ug\mL concentration with 10.16%. This extract has a toxic substance but does not exceed the toxicity threshold which achieves significant anti-inflammatory activity with depandant doses.
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Abdeddaim, S., Moussaoui, A., Boudjema, A., Kacimi, M., Merah, O., Morsli, A., Benali, M., 2021. Evaluation of in vitro biological activities of Pistacia lentiscus L. from Algeria.Journal of Ethnic Foods, 8, 21. doi: 10.1186/s42779-021-00091-2
Alhazmi, H.A., Najmi, A., Javed, S.A., Sultana, S., Al Bratty, M., Makeen, H. A., ... Khalid, A., 2021. Medicinal plants and isolated molecules demonstrating immunomodulation activity are potential alternative therapies for viral diseases, including COVID-19. Frontiers in immunology, 12, 637553. https://doi.org/10.3389/fimmu.2021.637553
Ali, W., Zhang, H., Junaid, M., Mao, K., Xu, N., Chang, C., ... Yang, Z., 2021. Insights into the mechanisms of arsenic-selenium interactions and the associated toxicity in plants, animals, and humans: A critical review. Critical reviews in environmental science and technology, 51(7), 704-750. https://doi.org/10.1080/10643389.2020.1740042
Benouali, A., Labdouni, M., Redhaoui, S., 2023. Evaluation of the antioxidant, antimicrobial, hemolytic and anti-inflammatory activities of Capparis spinosa. dspace.univ-tiaret.dz/bitstream/123456789/13248/1/TH.M.SNV.2023.61.pdf
Berger, K., 2022. Einfluss ausgewählter roter Fruchtsaftextrakte und deren Inhaltsstoffe auf den Glucosestoffwechsel in vitro und in vivo. http://dx.doi.org/10.1002/lemi.202352222
Bharadwaj, M., Mondal, B., Lata, M., 2021. Scope of utilization of tannin and saponin to improve animal performance. J. Entomol. Zool. Stud. http://dspace.univ-tiaret.dz/bitstream/123456789/13248/1/TH.M.SNV.2023.61.pdf
Bindu, S., Mazumder, S., Bandyopadhyay, U., 2020. Non-steroidal anti-inflammatory drugs (NSAIDs) and organ damage: A current perspective. Biochemical pharmacology. https://doi.org/10.1016%2Fj.bcp.2020.114147
Boubaker, J., Skandrani, I., Limem, I., Bhouri, W., Neffati, A., Sghaier, M.B., Bouhlel, I., Kilani, S., Ghedira, K., Chekir-Ghedira, L., 2009. Pistacia lentiscus L. essential oil inhibits the inflammatory response of human monocytes through differential regulation of NF-κB and MAPK signaling pathways. Journal of Ethnopharmacology, 126(3), 311-318. doi: 10.1016/j.jep.2009.08.025
Bouhlali, E.D., Bammou, M., Sellam, K., Benlyas, M., Alem, C., 2016. Antioxidant and anti-inflammatory properties of eleven Moroccan date fruit (Phoenix dactylifera L.) varieties. Journal of King Saud University-Science., 28(3), 235-244.
Boukeloua, A., 2012. Antioxidant and antibacterial activities of Pistacia lentiscus L. leaves extracts. Afr J Pharm Pharmacol., 6(11), 789-795. doi: 10.5897/ajpp11.612
Bourroubey, B., Chelli, N., Touil, A. T., Meddah, B., 2023. Ethnobotanical Survey, Phytochemical Screening and Antioxidant Activity of Methanolic Extracts of Pistacia lentiscus L. Growing in Northwestern Algeria. French-Ukrainian Journal of Chemistry, 11(1), 1-16. https://doi.org/10.17721/fujcV11I1P1-16
Bouzenna, H., Hfaiedh, N., Ben Slima, S., 2016. In vitro evaluation of antioxidant and anti-inflammatory activities of Pistacia lentiscus leaf and fruit extracts. J Med Food.;19(6):572-577. doi:10.1089/jmf.2016.0010
Buljeta, I., Pichler, A., Šimunović, J., Kopjar, M., 2023. Beneficial effects of red wine polyphenols on human health: comprehensive review. Current issues in molecular biology, 45(2), 782-798. http://dx.doi.org/10.3390/cimb45020052
Caro-Ordieres, T., Marín-Royo, G., Opazo-Ríos, L., Jiménez-Castilla, L., Moreno, J. A., Gómez-Guerrero, C., Egido, J., 2020. The coming age of flavonoids in the treatment of diabetic complications. Journal of clinical medicine, 9(2), 346. http://dx.doi.org/10.3390/jcm9020346
Cavalcanti, J.L.M.B., Santos, L.G.P.D., Alves, J.V.D.O., Almeida, W.A.D., Coutinho, G.D.., Araújo, A.M.S., ... Pontual, E.V., 2024. Evaluation of Antioxidant, Antibacterial, and Anti-hemolytic Properties of Ethanol Extract from Plectranthus barbatus Andrews (Lamiaceae) Leaves. Advances in Research, 25(5), 120-130. http://dx.doi.org/10.9734/air/2024/v25i51143
Chandra, S., Chatterjee, P., Dey, P., Bhattacharya, S., 2012. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine. 2 (1) Supplement. P S178-S180. ISSN 2221-1691. https://doi.org/10.1016/S2221-1691(12)60154-3.
Checkouri, E., Reignier, F., Robert-Da Silva, C., Meilhac, O., 2020. Evaluation of Polyphenol Content and Antioxidant Capacity of Aqueous Extracts from Eight Medicinal Plants from Reunion Island: Protection against Oxidative Stress in Red Blood Cells and Preadipocytes. Antioxidants, 9(10), 959. http://dx.doi.org/10.3390/antiox9100959
Cherbal, A., Hireche, S., Kasabri, V., Alawi, S. H., Afifi, F. U., Abaza, I., ... Madani, K., 2022. Pancreatic lipase inhibitory and antiproliferative effects of Olea europaea L., Pistacia Lentiscus L. and Marrubiu vulgare on obesity-related human colorectal cancer cell lines. Int. J. Nat. Eng. Sci, 16, 137-154. https://www.researchgate.net/publication/370278732
D’Aquila, P., Giacconi, R., Malavolta, M., Piacenza, F., Bürkle, A., Villanueva, M. M., ... Bellizzi, D., 2021. Microbiome in blood samples from the general population recruited in the MARK-AGE project: a pilot study. Frontiers in microbiology, 12, 707515. https://doi.org/10.3389/fmicb.2021.707515
Drioiche, A., Ailli, A., Remok, F., Saidi, S., Gourich, A.A., Asbabou, A., ... Zair, T., 2023. Analysis of the Chemical Composition and Evaluation of the Antioxidant, Antimicrobial, Anticoagulant, and Antidiabetic Properties of Pistacia lentiscus from Boulemane as a Natural Nutraceutical Preservative. Biomedicines, 11(9), 2372. https://doi.org/10.3390/biomedicines11092372
Elez G., I., Kruk, V., Martić, A., Martić, I., Zorić, Z., Pedisić, S., ... Dragović-Uzelac, V., 2020. Evaluation of polyphenolic profile and antioxidant activity of Pistacia lentiscus L. leaves and fruit extract obtained by optimized microwave-assisted extraction. Foods, 9(11), 1556. https://doi.org/10.3390/foods9111556
Enyoh, C.E., Shafea, L., Verla, A.W., Verla, E.N., Qingyue, W., Chowdhury, T., Paredes, M., 2020. Microplastics exposure routes and toxicity studies to ecosystems: an overview. Environmental analysis, health and toxicology, 35(1). https://doi.org/10.5620%2Feaht.e2020004
Fraga-Corral, M., Otero, P., Echave, J., Garcia-Oliveira, P., Carpena, M., Jarboui, A., ... Prieto, M.A., 2021. By-products of agri-food industry as tannin-rich sources: A review of tannins’ biological activities and their potential for valorization. Foods, 10(1), 137. http://dx.doi.org/10.3390/foods10010137
Guo-Xiang, L., Zai-qun, L., 2007. The protective effects of ginsenosides on human erythrocytes againts hemin-induced hemolysis. Food and chemical Toxicology 46:886-892.
Islam, S., Fahad, F.I., Sultana, A., Sayem, S.A.J., Roy, S.B., Islam, M.N., ... Sayeed, M.A., 2022. Evaluation of Antioxidant, Cytotoxic, Anti‐Inflammatory, Antiarthritic, Thrombolytic, and Anthelmintic Activity of Methanol Extract of Lepidagathis hyalina Nees Root. Evidence‐Based Complementary and Alternative Medicine, 2022(1), 2515260. https://doi.org/10.1155/2022/2515260
Jahanban-Esfahlan, A., Modaeinama, S., Abasi, M., Abbasi, M.M., 2017. Pistacia lentiscus extract attenuates mechanical hyperalgesia in a rat model of neuropathic pain: involvement of the opioid system. Avicenna Journal of Phytomedicine, 7(6), 521-529. PMID: 29184685.
Jiang, W., Tang, M., Yang, L., Zhao, X., Gao, J., Jiao, Y., ... Jiang, J.D., 2022. Analgesic alkaloids derived from traditional Chinese medicine in pain management. Frontiers in Pharmacology, 13, 851508. https://doi.org/10.3389/fphar.2022.851508
Kar, B., Suresh Kumar, R.B., Karmakar, I., Dola, N., Bala, A., Mazumder, U.K., Hadar, P.K., 2012. Antioxidant and in vitro anti-inflammatory activities of Mimusops elengi leaves. Asian Pacific Journal of Tropical Biomedicine. 2 (2) Supplement. S976-S980. https://doi.org/10.1016/S2221-1691(12)60346-3.
Kejík, Z., Kaplánek, R., Masařík, M., Babula, P., Matkowski, A., Filipenský, P., ... Jakubek, M., 2021. Iron complexes of flavonoids-antioxidant capacity and beyond. International journal of molecular sciences, 22(2), 646. http://dx.doi.org/10.3390/ijms22020646
Khan, M.S.H., Hegde, V., 2020. Obesity and diabetes mediated chronic inflammation: a potential biomarker in Alzheimer's disease. Journal of personalized medicine. J. Pers. Med. 10(2), 42. https://doi.org/10.3390/jpm10020042
Leszek, J., Mikhaylenko, E.V., Belousov, D.M., Koutsouraki, E., Szczechowiak, K., Kobusiak-Prokopowicz, M., ... Aliev, G., 2021. The links between cardiovascular diseases and Alzheimer's disease. Current Neuropharmacology, 19(2), 152-169. https://doi.org/10.2174%2F1570159X18666200729093724
Li, Q., Tian, C., Liu, X., Li, D., Liu, H., 2023. Anti-inflammatory and antioxidant traditional Chinese Medicine in treatment and prevention of osteoporosis. Frontiers in Pharmacology, 14, https://doi.org/10.3389/fphar.2023.1203767
Li, W., Yu, L., Li, W., Ge, G., Ma, Y., Xiao, L., ... Geng, D., 2023. Prevention and treatment of inflammatory arthritis with traditional Chinese medicine: underlying mechanisms based on cell and molecular targets. Ageing Research Reviews, 89, 101981. https://doi.org/10.1016/j.arr.2023.101981
Madakka, M., Jayaraju, N., Rajesh, N., 2021. Evaluating the antimicrobial activity and antitumor screening of green synthesized silver nanoparticles compounds, using Syzygium jambolanum, towards MCF7 cell line (Breast cancer cell line). Journal of Photochemistry and Photobiology, 6, 100028. https://doi.org/10.1016/j.jpap.2021.100028
Milia, E., Bullitta, S.M., Mastandrea, G., Szotáková, B., Schoubben, A., Langhansová, L., ... Eick, S., 2021. Leaves and fruits preparations of Pistacia lentiscus L.: a review on the ethnopharmacological uses and implications in inflammation and infection. Antibiotics, 10(4), 425. https://doi.org/10.3390/antibiotics10040425
Nunes, C.D.R., Barreto Arantes, M., Menezes de Faria Pereira, S., Leandro da Cruz, L., de Souza Passos, M., Pereira de Moraes, L., ... Barros de Oliveira, D., 2020. Plants as sources of anti-inflammatory agents. Molecules, 25(16), 3726. https://doi.org/10.3390/molecules25163726
Olchowik-Grabarek, E., Sekowski, S., Bitiucki, M., Dobrzynska, I., Shlyonsky, V., Ionov, M., ... Zamaraeva, M., 2020. Inhibition of interaction between Staphylococcus aureus α-hemolysin and erythrocytes membrane by hydrolysable tannins: structure-related activity study. Scientific reports, 10(1), 11168. http://dx.doi.org/10.1038/s41598-020-68030-1
Olchowik-Grabarek, E., Sekowski, S., Mies, F., Bitiucki, M., Swiecicka, I., Abdulladjanova, N., ... Zamaraeva, M., 2023. Electrophysiological and spectroscopic investigation of hydrolysable tannins interaction with α-hemolysin of S. aureus. Bioelectrochemistry, 150, 108318. http://dx.doi.org/10.1016/j.bioelechem.2022.108318
Ooi, S.L., Pak, S.C., Campbell, R., Manoharan, A., 2022. Polyphenol-Rich Ginger (Zingiber officinale) for Iron Deficiency Anaemia and Other Clinical Entities Associated with Altered Iron Metabolism. Molecules. http://dx.doi.org/10.3390/molecules27196417
Purba, R.A.P., Paengkoum, P.F., 2022. Farang (Psidium guajava L.) dried leaf extracts: Phytochemical profiles, antioxidant, anti-diabetic, and anti-hemolytic properties for ruminant health and production. Molecules, 27(24), 8987. http://dx.doi.org/10.3390/molecules27248987
Sabrina, B., Daoud, H., Amina, Z., Nouari, S., Asma, B., Soufiane, G., Oumaima, N., 2020. Antimicrobial and Antioxidant Activities of Flavonoids Extracted from Pistacia lentiscus L., Leaves. Journal of Drug Delivery and Therapeutics, 10. https://doi.org/10.22270/jddt.v10i1-s.3895
Salmerón-Manzano, E., Garrido-Cardenas, J. A., and Manzano-Agugliaro, F., 2020. Worldwide research trends on medicinal plants. International journal of environmental research and public health, 17(10), 3376. https://doi.org/10.3390/ijerph17103376
Süntar, I., 2020. Importance of ethnopharmacological studies in drug discovery: role of medicinal plants. Phytochemistry Reviews. https://doi.org/10.1007/s11101-019-09629-9
Tanaka, N., Kashiwada, Y., 2021. Phytochemical studies on traditional herbal medicines based on the ethnopharmacological information obtained by field studies. J Nat Med. 2021 Sep;75(4):762-783. doi: 10.1007/s11418-021-01545-7.
Tebbi, S.O., Trapali, M., Letsiou, S., 2024. Exploring the Anti-Diabetic, Antioxidant and Antimicrobial Properties of Clematis flammula L. Leaves and Pistacia lentiscus L. Fruits Using Choline Chloride-Based Eutectic Solvent. Waste and Biomass Valorization, 15, 2869-2879. http://dx.doi.org/10.1007/s12649-023-02360-9
Tedesco, I., Spagnuolo, C., Russo, G. L., Russo, M., Cervellera, C., Moccia, S., 2021. The pro-oxidant activity of red wine polyphenols induces an adaptive antioxidant response in human erythrocytes. Antioxidants, 10(5), 800. http://dx.doi.org/10.3390/antiox10050800
Thiruchenthooran, V., Sánchez-López, E., Gliszczyńska, A., 2023. Perspectives of the application of non-steroidal anti-inflammatory drugs in cancer therapy: Attempts to overcome their unfavorable side effects. Cancers, 15(2), 475. https://doi.org/10.3390/cancers15020475
Tini, G., Scagliola, R., Monacelli, F., La Malfa, G., Porto, I., Brunelli, C., Rosa, G.M., 2020. Alzheimer’s disease and cardiovascular disease: a particular association. Cardiology research and practice, 2020(1), 2617970. https://doi.org/10.1155/2020/2617970
Williams, R.O., Feldmann, M., Maini, R.N., 2008. Anti-TNF therapy: past, present and future. International Immunology, 20(7), 813-818.
Wiszniewska, A., 2021. Priming strategies for benefiting plant performance under toxic trace metal exposure. Plants 10(4), 623. https://doi.org/10.3390/plants10040623
Zam, W., Ali, A., Hasan, R., 2020. Determination of phenolic compounds' extraction conditions from Pistacia palaestina leaves at two different stages of maturity. Current Nutrition and Food Science, 16(5), 808-814. http://dx.doi.org/10.2174/1573401315666191009100726
Zhan, J., Liu, Q. S., Zhang, Y., Sun, Z., Zhou, Q., Jiang, G., 2023. Silica nanoparticles trigger phosphatidylserine exposure in red blood cells and induce thrombosis risk. Environmental Pollution, 327, 121591. https://doi.org/10.1016/j.envpol.2023.121591
Zitouni, A., Chekroun-Bechlaghem, N., Ghembaza, N., Belyagoubi-Benhammou, N., 2023. Lentisk fruits (Pistacia lentiscus L.) as sources of phytochemicals with potential health benefits: A review. Journal of Natural Product Research and Applications, 3(01), 27-44. https://10.46325/jnpra.v3i01.50
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