Designer cellulosomes – catalytic nanomachines with significant potential in biotechnology and circular economy

Maša Vodovnik

doi:10.14720/abs.68.01.21452

Authors

Maša Vodovnik University of Ljubljana, Biotechnical Faculty, Department of Microbiology, Chair of Microbiomics, Diversity and Biotechnology, Jamnikarjeva 101, 1000 Ljubljana, Slovenia

DOI:

https://doi.org/10.14720/abs.68.01.21452

Keywords:

Designer cellulosomes, lignocellulose valorization, protein engineering, nanobiotechnology, bio-based products

Abstract

Cellulosomes are multienzyme complexes originally found on the surface of certain anaerobic celluloytic bacteria and fungi that are specialized in the degradation of plant cell walls. Recently, the efficiency in lignocellulose conversion and architectural features of these intricate complexes inspired the construction of artificial chimeric complexes for targeted substrate degradation. The simultaneous advancements in synthetic biology, protein engineering, and the pursuit of greater sustainability across various industries have highlighted the immense potential of these artificially designed enzymatic complexes for diverse applications. Notably, they hold significant promise for industries specializing in valorization of plant biomass waste and the production of bio-based renewable energy. The article discusses the main architectural features, design and construction steps as well as various biotechnological applications of these intriguing nanomachines.

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References

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