Mikropropagacija vrste Nephrolepis exaltata

Authors

  • Marjana Camloh
  • Nada Gogala
  • Romana Ružič

DOI:

https://doi.org/10.14720/abs.37.3.23304

Keywords:

Nephrolepis exaltata, tissue culture, micropropagation

Abstract

The micropropagation of Nephro/epis exaltata with runner tips as primary explants is described. The influence of 0.5, 2.5, 5 or 15µM 2iP added to the modified MS medium (BM) on shoot formation in primary explants was tested. A concentration of 0.5µM 2iP gave the highest number and fresh weight of the shoots. The effect of different growth regulators and dilutions of BM on shoot de­ velopment in secondary explants separated from the primary explants was studied. On 1/2-strength BM containing 0.5µM NAA and 5µM KIN the highest number of shoots was obtained. To induce rooting, the shoots were transferred to a full-strength or diluted BM, hormone-free or supplemented with 6 or 12µM IAA. The highest number of roots and the highest percentage of the shoots that formed roots were obtained on a 1/2-strength hormone-free BM.

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References

BECK M J, CAPONEITI J D 1983 The effect of kinetin and naphthaleneacetic acid on in vitro shoot multiplication and rooting in the fishtail fern. Am. J. Bot., 70(1): 1-7.

CAPONETTI J D, BYRNE T E 1985 Morphogenesis in Boston ferns by tissue culture. Am. J. Bot., 72: 920.

COOKER C 1977 The use of an agar substitute in the initial growth of Boston ferns in vitro. Hort Science, 12(4): 339.

LOESCHER W H, ALBRECHT C N 1979 Development in vitro of Nephrolepis exaltata cv. Bostoniensis runner tisues. Physiol. Plant., 47: 250-254.

MURASHIGE T 1975 Plant propagation trough tissue cultures. Annu. Rev. Plant Physiol., 25: 135-166.

PADHYA M A, MEHTA A R 1982 Propagation of fern (Nephrolepis) through tissue culture. Plant Cell Reports, I: 261-263.

PAEK KY, LEECH, CHOI J K, KWACK B H, 1984 Masspropagation of Nephrolepis exaltata by runner tips in vitro. J. Kor. Soc. Horti Sci., 25(4): 313-321.

THEILER-HEDTRICH C M 1983 Nephrolepis exaltata Neuzeitliche Produktion aus Gewebekultur. Gb + Gw, 23: 572-573.

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Published

16.07.1989

Issue

Vol. 37 No. 3 (1989): Biološki vestnik

Section

Original Research Paper

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

How to Cite

Camloh, M., Gogala, N., & Ružič, R. (1989). Mikropropagacija vrste Nephrolepis exaltata . Acta Biologica Slovenica, 37(3), 23-32. https://doi.org/10.14720/abs.37.3.23304
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